Evidence for extensive distribution of oestrogen receptor α-immunoreactivity in the cerebral cortex of adult rats

J. A. Butler, I. Kalló, M. Sjöberg, Clive W. Coen

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The presence of oestrogen receptor alpha (ERα)-immunoreactivity (IR) in the cerebral cortex of adult rats has been investigated. Previous studies have reported a negligible presence of ERα or ERα mRNA in this region of the adult rat brain. We have used immunoprecipitation and immunohistochemistry, with various antibodies and fixatives, to detect this protein in the cingulate cortex. When the tissue was fixed using paraformaldehyde alone only faint ERα-IR was observed at this site. In contrast, following fixation either with acrolein (with or without paraformaldehyde) or with a mixture of paraformaldehyde and glutaraldehyde there was extensive ERα-IR throughout layers II to VI; this was absent when the antibodies were preincubated with the peptide fragment used in their production. The presence of ERα-IR in the nonfixed cingulate cortex of adult rats was confirmed by immunoprecipitation.

Original languageEnglish
Pages (from-to)325-329
Number of pages5
JournalJournal of Neuroendocrinology
Issue number5
Publication statusPublished - May 7 1999



  • Cerebral cortex
  • Immunohistochemistry
  • Immunoprecipitation
  • Oestrogen receptor
  • Rats

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology
  • Endocrine and Autonomic Systems
  • Cellular and Molecular Neuroscience

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