Evidence for a megareplicon covering megabases of centromeric chromosome segments

Gy Holló, J. Keresõ, T. Praznovszky, I. Cserpán, K. Fodor, R. Katona, E. Csonka, K. Fátyol, A. Szeles, A. A. Szalay, Gy Hadlaczky

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

We have analysed the replication of the heterochromatic megachromosome that was formed de novo by a large scale amplification process initiated in the centromeric region of mouse chromosome 7. The megachromosome is organized into amplicons ~30 Mb in size, and each amplicon consists of two large inverted repeats delimited by a primary replication initiation site. Our results suggest that these segments represent a higher order replication unit (megareplicon) of the centromeric region of mouse chromosomes. Analysis of the replication of the megareplicons indicates that the pericentric. heterochromatin and the centromere of mouse chromosomes begin to replicate early, and that their replication continues through approximately three-quarters of the S-phase. We suggest that a replication-directed mechanism may account for the initiation of large-scale amplification in the centromeric regions of mouse chromosomes, and may also explain the formation of new, stable chromosome segments and chromosomes.

Original languageEnglish
Pages (from-to)240-247
Number of pages8
JournalChromosome Research
Volume4
Issue number3
DOIs
Publication statusPublished - 1996

Fingerprint

Chromosomes
Replicon
Chromosomes, Human, Pair 7
Heterochromatin
Centromere
S Phase

Keywords

  • Amplification
  • Centromere
  • Heterochromatin
  • Megareplicon
  • Replication

ASJC Scopus subject areas

  • Genetics

Cite this

Holló, G., Keresõ, J., Praznovszky, T., Cserpán, I., Fodor, K., Katona, R., ... Hadlaczky, G. (1996). Evidence for a megareplicon covering megabases of centromeric chromosome segments. Chromosome Research, 4(3), 240-247. https://doi.org/10.1007/BF02254965

Evidence for a megareplicon covering megabases of centromeric chromosome segments. / Holló, Gy; Keresõ, J.; Praznovszky, T.; Cserpán, I.; Fodor, K.; Katona, R.; Csonka, E.; Fátyol, K.; Szeles, A.; Szalay, A. A.; Hadlaczky, Gy.

In: Chromosome Research, Vol. 4, No. 3, 1996, p. 240-247.

Research output: Contribution to journalArticle

Holló, G, Keresõ, J, Praznovszky, T, Cserpán, I, Fodor, K, Katona, R, Csonka, E, Fátyol, K, Szeles, A, Szalay, AA & Hadlaczky, G 1996, 'Evidence for a megareplicon covering megabases of centromeric chromosome segments', Chromosome Research, vol. 4, no. 3, pp. 240-247. https://doi.org/10.1007/BF02254965
Holló, Gy ; Keresõ, J. ; Praznovszky, T. ; Cserpán, I. ; Fodor, K. ; Katona, R. ; Csonka, E. ; Fátyol, K. ; Szeles, A. ; Szalay, A. A. ; Hadlaczky, Gy. / Evidence for a megareplicon covering megabases of centromeric chromosome segments. In: Chromosome Research. 1996 ; Vol. 4, No. 3. pp. 240-247.
@article{8b72c365e01b414c9976884088290dad,
title = "Evidence for a megareplicon covering megabases of centromeric chromosome segments",
abstract = "We have analysed the replication of the heterochromatic megachromosome that was formed de novo by a large scale amplification process initiated in the centromeric region of mouse chromosome 7. The megachromosome is organized into amplicons ~30 Mb in size, and each amplicon consists of two large inverted repeats delimited by a primary replication initiation site. Our results suggest that these segments represent a higher order replication unit (megareplicon) of the centromeric region of mouse chromosomes. Analysis of the replication of the megareplicons indicates that the pericentric. heterochromatin and the centromere of mouse chromosomes begin to replicate early, and that their replication continues through approximately three-quarters of the S-phase. We suggest that a replication-directed mechanism may account for the initiation of large-scale amplification in the centromeric regions of mouse chromosomes, and may also explain the formation of new, stable chromosome segments and chromosomes.",
keywords = "Amplification, Centromere, Heterochromatin, Megareplicon, Replication",
author = "Gy Holl{\'o} and J. Keres{\~o} and T. Praznovszky and I. Cserp{\'a}n and K. Fodor and R. Katona and E. Csonka and K. F{\'a}tyol and A. Szeles and Szalay, {A. A.} and Gy Hadlaczky",
year = "1996",
doi = "10.1007/BF02254965",
language = "English",
volume = "4",
pages = "240--247",
journal = "Chromosome Research",
issn = "0967-3849",
publisher = "Springer Netherlands",
number = "3",

}

TY - JOUR

T1 - Evidence for a megareplicon covering megabases of centromeric chromosome segments

AU - Holló, Gy

AU - Keresõ, J.

AU - Praznovszky, T.

AU - Cserpán, I.

AU - Fodor, K.

AU - Katona, R.

AU - Csonka, E.

AU - Fátyol, K.

AU - Szeles, A.

AU - Szalay, A. A.

AU - Hadlaczky, Gy

PY - 1996

Y1 - 1996

N2 - We have analysed the replication of the heterochromatic megachromosome that was formed de novo by a large scale amplification process initiated in the centromeric region of mouse chromosome 7. The megachromosome is organized into amplicons ~30 Mb in size, and each amplicon consists of two large inverted repeats delimited by a primary replication initiation site. Our results suggest that these segments represent a higher order replication unit (megareplicon) of the centromeric region of mouse chromosomes. Analysis of the replication of the megareplicons indicates that the pericentric. heterochromatin and the centromere of mouse chromosomes begin to replicate early, and that their replication continues through approximately three-quarters of the S-phase. We suggest that a replication-directed mechanism may account for the initiation of large-scale amplification in the centromeric regions of mouse chromosomes, and may also explain the formation of new, stable chromosome segments and chromosomes.

AB - We have analysed the replication of the heterochromatic megachromosome that was formed de novo by a large scale amplification process initiated in the centromeric region of mouse chromosome 7. The megachromosome is organized into amplicons ~30 Mb in size, and each amplicon consists of two large inverted repeats delimited by a primary replication initiation site. Our results suggest that these segments represent a higher order replication unit (megareplicon) of the centromeric region of mouse chromosomes. Analysis of the replication of the megareplicons indicates that the pericentric. heterochromatin and the centromere of mouse chromosomes begin to replicate early, and that their replication continues through approximately three-quarters of the S-phase. We suggest that a replication-directed mechanism may account for the initiation of large-scale amplification in the centromeric regions of mouse chromosomes, and may also explain the formation of new, stable chromosome segments and chromosomes.

KW - Amplification

KW - Centromere

KW - Heterochromatin

KW - Megareplicon

KW - Replication

UR - http://www.scopus.com/inward/record.url?scp=8944238472&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=8944238472&partnerID=8YFLogxK

U2 - 10.1007/BF02254965

DO - 10.1007/BF02254965

M3 - Article

VL - 4

SP - 240

EP - 247

JO - Chromosome Research

JF - Chromosome Research

SN - 0967-3849

IS - 3

ER -