Evaluation of the MDR1, ABCG2, Topoisomerases IIα and GSTπ gene expression in patients affected by aggressive mantle cell lymphoma treated by the R-Hyper-CVAD regimen

Sara Galimberti, Balint Nagy, Edoardo Benedetti, Simone Pacini, Stefania Brizzi, Francesco Caracciolo, Federico Papineschi, Elena Ciabatti, Francesca Guerrini, Rita Fazzi, Martina Canestraro, Mario Petrini

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9 Citations (Scopus)

Abstract

The genomic profile of mantle cell lymphoma (MCL) has been reported to be significantly different from that of other indolent lymphoproliferative disorders, Topoisomerase IIα, glutathione-s-transferaseπ (GSTπ) and ABCG2 (BCRP) chemoresistance genes being over-expressed in MCL. In our study, expression levels of the above mentioned genes plus MDR1 were tested on bone marrow samples from 20 patients treated with Rituximab plus hyper-CVAD regimen, in order to evaluate a possible impact of the chemoresistance phenomenon on this promising treatment regimen. All patients expressed ABCG2 and MDR1 genes; 85% of cases expressed GSTπ and topoisomerase IIα. Only ABCG2 were over-expressed in comparison both with marrow from healthy donors and tonsilar CD5+/CD20+ lymphocytes (adopted as normal counterpart of the neoplastic population). The overall response rate of the entire series was 87.5%, with 44% of complete responses. Fifty-seven percent of patients achieved the clearance of minimal residual disease. Levels of tested genes did not condition either quality of clinical response or PFS (76% at 24 months). Nevertheless, an ABCG2 higher expression appeared associated with a worse PFS and levels of this gene paralleled the status of minimal residual disease. A further evaluation of ABCG2 expression in larger series of MCL patients would be suitable.

Original languageEnglish
Pages (from-to)1502-1509
Number of pages8
JournalLeukemia and Lymphoma
Volume48
Issue number8
DOIs
Publication statusPublished - Aug 1 2007

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Keywords

  • ABCG2
  • GSTπ
  • Hyper-CVAD
  • MDR1
  • Mantle cell lymphoma
  • Rituximab
  • Topisomerase IIα

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Cancer Research

Cite this

Galimberti, S., Nagy, B., Benedetti, E., Pacini, S., Brizzi, S., Caracciolo, F., Papineschi, F., Ciabatti, E., Guerrini, F., Fazzi, R., Canestraro, M., & Petrini, M. (2007). Evaluation of the MDR1, ABCG2, Topoisomerases IIα and GSTπ gene expression in patients affected by aggressive mantle cell lymphoma treated by the R-Hyper-CVAD regimen. Leukemia and Lymphoma, 48(8), 1502-1509. https://doi.org/10.1080/10428190701402895