Evaluation of the Biolog system for the identification of certain closely related Pasteurella species

Boglárka Sellyei, E. Wehmann, L. Makrai, Tibor Magyar

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The zoonotic impact of Pasteurella species in human wounds caused by cats and dogs has increased recently. In this study, the effectiveness of the Biolog Microstation ID System (Biolog, Hayward, CA) for the identification of certain species of Pasteurella sensu stricto was analysed. Thirty-eight isolates originating from dogs and cats were studied by pheno- and genotypic methods. The classical biochemical tests identified these isolates as P. multocida, P. dagmatis, and P. canis, while the Biolog system distinguished only 2 categories: P. multocida and P. dagmatis. The sodA gene sequence-based phylogenetic analysis revealed that the isolates identified as P. dagmatis by the Biolog system were either P. dagmatis, P. canis, or P. dagmatis-like genomospecies. The low discrimination power of the Biolog system in the case of these closely related Pasteurella species draws attention to the need of continuously improving the database of automated microbial identification systems.

Original languageEnglish
Pages (from-to)6-11
Number of pages6
JournalDiagnostic Microbiology and Infectious Disease
Volume71
Issue number1
DOIs
Publication statusPublished - Sep 2011

Fingerprint

Pasteurella
Cats
Dogs
Zoonoses
Databases
Wounds and Injuries
Genes

Keywords

  • Biolog system
  • P. canis
  • P. dagmatis
  • P. multocida
  • SodA gene

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

Cite this

Evaluation of the Biolog system for the identification of certain closely related Pasteurella species. / Sellyei, Boglárka; Wehmann, E.; Makrai, L.; Magyar, Tibor.

In: Diagnostic Microbiology and Infectious Disease, Vol. 71, No. 1, 09.2011, p. 6-11.

Research output: Contribution to journalArticle

@article{9511ff9461914504b3f011a63743acf3,
title = "Evaluation of the Biolog system for the identification of certain closely related Pasteurella species",
abstract = "The zoonotic impact of Pasteurella species in human wounds caused by cats and dogs has increased recently. In this study, the effectiveness of the Biolog Microstation ID System (Biolog, Hayward, CA) for the identification of certain species of Pasteurella sensu stricto was analysed. Thirty-eight isolates originating from dogs and cats were studied by pheno- and genotypic methods. The classical biochemical tests identified these isolates as P. multocida, P. dagmatis, and P. canis, while the Biolog system distinguished only 2 categories: P. multocida and P. dagmatis. The sodA gene sequence-based phylogenetic analysis revealed that the isolates identified as P. dagmatis by the Biolog system were either P. dagmatis, P. canis, or P. dagmatis-like genomospecies. The low discrimination power of the Biolog system in the case of these closely related Pasteurella species draws attention to the need of continuously improving the database of automated microbial identification systems.",
keywords = "Biolog system, P. canis, P. dagmatis, P. multocida, SodA gene",
author = "Bogl{\'a}rka Sellyei and E. Wehmann and L. Makrai and Tibor Magyar",
year = "2011",
month = "9",
doi = "10.1016/j.diagmicrobio.2011.04.016",
language = "English",
volume = "71",
pages = "6--11",
journal = "Diagnostic Microbiology and Infectious Disease",
issn = "0732-8893",
publisher = "Elsevier Inc.",
number = "1",

}

TY - JOUR

T1 - Evaluation of the Biolog system for the identification of certain closely related Pasteurella species

AU - Sellyei, Boglárka

AU - Wehmann, E.

AU - Makrai, L.

AU - Magyar, Tibor

PY - 2011/9

Y1 - 2011/9

N2 - The zoonotic impact of Pasteurella species in human wounds caused by cats and dogs has increased recently. In this study, the effectiveness of the Biolog Microstation ID System (Biolog, Hayward, CA) for the identification of certain species of Pasteurella sensu stricto was analysed. Thirty-eight isolates originating from dogs and cats were studied by pheno- and genotypic methods. The classical biochemical tests identified these isolates as P. multocida, P. dagmatis, and P. canis, while the Biolog system distinguished only 2 categories: P. multocida and P. dagmatis. The sodA gene sequence-based phylogenetic analysis revealed that the isolates identified as P. dagmatis by the Biolog system were either P. dagmatis, P. canis, or P. dagmatis-like genomospecies. The low discrimination power of the Biolog system in the case of these closely related Pasteurella species draws attention to the need of continuously improving the database of automated microbial identification systems.

AB - The zoonotic impact of Pasteurella species in human wounds caused by cats and dogs has increased recently. In this study, the effectiveness of the Biolog Microstation ID System (Biolog, Hayward, CA) for the identification of certain species of Pasteurella sensu stricto was analysed. Thirty-eight isolates originating from dogs and cats were studied by pheno- and genotypic methods. The classical biochemical tests identified these isolates as P. multocida, P. dagmatis, and P. canis, while the Biolog system distinguished only 2 categories: P. multocida and P. dagmatis. The sodA gene sequence-based phylogenetic analysis revealed that the isolates identified as P. dagmatis by the Biolog system were either P. dagmatis, P. canis, or P. dagmatis-like genomospecies. The low discrimination power of the Biolog system in the case of these closely related Pasteurella species draws attention to the need of continuously improving the database of automated microbial identification systems.

KW - Biolog system

KW - P. canis

KW - P. dagmatis

KW - P. multocida

KW - SodA gene

UR - http://www.scopus.com/inward/record.url?scp=80051670425&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80051670425&partnerID=8YFLogxK

U2 - 10.1016/j.diagmicrobio.2011.04.016

DO - 10.1016/j.diagmicrobio.2011.04.016

M3 - Article

C2 - 21795002

AN - SCOPUS:80051670425

VL - 71

SP - 6

EP - 11

JO - Diagnostic Microbiology and Infectious Disease

JF - Diagnostic Microbiology and Infectious Disease

SN - 0732-8893

IS - 1

ER -