Evaluation of SV40 in osteosarcoma and healthy population

A Hungarian-German study

Susanne Heinsohn, M. Szendrői, Stefan Bielack, Udo Zur Stadt, Hartmut Kabisch

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Simian virus 40 (SV40) has been linked to human cancer, as has osteosarcoma (OS). Although significant discrepancies exist in the frequency, evidence that the virus plays a causative role in some malignancies is mounting. The large-T-antigen and significant SV40 oncoprotein, bind and inactivate the tumour suppressor genes p53 and pRb, which play a key role in osteosarcoma development. We analysed 277 OS samples from two different European countries (154 OS samples from Hungary and 123 from Germany). To ascertain the prevalence of SV40 in the general population, additional blood samples from healthy volunteers from the two countries (166 Hungarian and 149 German) were analysed. To reach an appropriate detection level, we investigated a real-time quantitative PCR-based assay for the detection and quantification of SV40 100 copies), whereas only 22% (29/123) of the German OS samples harbour small amounts of SV40 (∼10 copies). SV40 was detected in 8 of 60 German tumour samples (14%) and 21 of 63 German blood samples (33%) from OS patients. In the peripheral blood of healthy volunteers we found only weak SV40 positivity (

Original languageEnglish
Pages (from-to)289-297
Number of pages9
JournalOncology Reports
Volume21
Issue number2
DOIs
Publication statusPublished - 2009

Fingerprint

Simian virus 40
Osteosarcoma
Population
Healthy Volunteers
Neoplasms
Hungary
Oncogene Proteins
Viral Tumor Antigens
Tumor Suppressor Genes
Germany
Real-Time Polymerase Chain Reaction
Viruses

Keywords

  • Large T-antigen
  • Osteosarcoma
  • Real-time quantitative PCR
  • Simian virus 40

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Evaluation of SV40 in osteosarcoma and healthy population : A Hungarian-German study. / Heinsohn, Susanne; Szendrői, M.; Bielack, Stefan; Stadt, Udo Zur; Kabisch, Hartmut.

In: Oncology Reports, Vol. 21, No. 2, 2009, p. 289-297.

Research output: Contribution to journalArticle

Heinsohn, Susanne ; Szendrői, M. ; Bielack, Stefan ; Stadt, Udo Zur ; Kabisch, Hartmut. / Evaluation of SV40 in osteosarcoma and healthy population : A Hungarian-German study. In: Oncology Reports. 2009 ; Vol. 21, No. 2. pp. 289-297.
@article{1cfe2616effb4371917320af26be9050,
title = "Evaluation of SV40 in osteosarcoma and healthy population: A Hungarian-German study",
abstract = "Simian virus 40 (SV40) has been linked to human cancer, as has osteosarcoma (OS). Although significant discrepancies exist in the frequency, evidence that the virus plays a causative role in some malignancies is mounting. The large-T-antigen and significant SV40 oncoprotein, bind and inactivate the tumour suppressor genes p53 and pRb, which play a key role in osteosarcoma development. We analysed 277 OS samples from two different European countries (154 OS samples from Hungary and 123 from Germany). To ascertain the prevalence of SV40 in the general population, additional blood samples from healthy volunteers from the two countries (166 Hungarian and 149 German) were analysed. To reach an appropriate detection level, we investigated a real-time quantitative PCR-based assay for the detection and quantification of SV40 100 copies), whereas only 22{\%} (29/123) of the German OS samples harbour small amounts of SV40 (∼10 copies). SV40 was detected in 8 of 60 German tumour samples (14{\%}) and 21 of 63 German blood samples (33{\%}) from OS patients. In the peripheral blood of healthy volunteers we found only weak SV40 positivity (",
keywords = "Large T-antigen, Osteosarcoma, Real-time quantitative PCR, Simian virus 40",
author = "Susanne Heinsohn and M. Szendrői and Stefan Bielack and Stadt, {Udo Zur} and Hartmut Kabisch",
year = "2009",
doi = "10.3892/or_00000220",
language = "English",
volume = "21",
pages = "289--297",
journal = "Oncology Reports",
issn = "1021-335X",
publisher = "Spandidos Publications",
number = "2",

}

TY - JOUR

T1 - Evaluation of SV40 in osteosarcoma and healthy population

T2 - A Hungarian-German study

AU - Heinsohn, Susanne

AU - Szendrői, M.

AU - Bielack, Stefan

AU - Stadt, Udo Zur

AU - Kabisch, Hartmut

PY - 2009

Y1 - 2009

N2 - Simian virus 40 (SV40) has been linked to human cancer, as has osteosarcoma (OS). Although significant discrepancies exist in the frequency, evidence that the virus plays a causative role in some malignancies is mounting. The large-T-antigen and significant SV40 oncoprotein, bind and inactivate the tumour suppressor genes p53 and pRb, which play a key role in osteosarcoma development. We analysed 277 OS samples from two different European countries (154 OS samples from Hungary and 123 from Germany). To ascertain the prevalence of SV40 in the general population, additional blood samples from healthy volunteers from the two countries (166 Hungarian and 149 German) were analysed. To reach an appropriate detection level, we investigated a real-time quantitative PCR-based assay for the detection and quantification of SV40 100 copies), whereas only 22% (29/123) of the German OS samples harbour small amounts of SV40 (∼10 copies). SV40 was detected in 8 of 60 German tumour samples (14%) and 21 of 63 German blood samples (33%) from OS patients. In the peripheral blood of healthy volunteers we found only weak SV40 positivity (

AB - Simian virus 40 (SV40) has been linked to human cancer, as has osteosarcoma (OS). Although significant discrepancies exist in the frequency, evidence that the virus plays a causative role in some malignancies is mounting. The large-T-antigen and significant SV40 oncoprotein, bind and inactivate the tumour suppressor genes p53 and pRb, which play a key role in osteosarcoma development. We analysed 277 OS samples from two different European countries (154 OS samples from Hungary and 123 from Germany). To ascertain the prevalence of SV40 in the general population, additional blood samples from healthy volunteers from the two countries (166 Hungarian and 149 German) were analysed. To reach an appropriate detection level, we investigated a real-time quantitative PCR-based assay for the detection and quantification of SV40 100 copies), whereas only 22% (29/123) of the German OS samples harbour small amounts of SV40 (∼10 copies). SV40 was detected in 8 of 60 German tumour samples (14%) and 21 of 63 German blood samples (33%) from OS patients. In the peripheral blood of healthy volunteers we found only weak SV40 positivity (

KW - Large T-antigen

KW - Osteosarcoma

KW - Real-time quantitative PCR

KW - Simian virus 40

UR - http://www.scopus.com/inward/record.url?scp=62849088644&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=62849088644&partnerID=8YFLogxK

U2 - 10.3892/or_00000220

DO - 10.3892/or_00000220

M3 - Article

VL - 21

SP - 289

EP - 297

JO - Oncology Reports

JF - Oncology Reports

SN - 1021-335X

IS - 2

ER -