Evaluation of regeneration capacity in grape towards the improvement of new cultivars with enhanced berry and wine quality

E. Novák, A. Zok, I. Forgács, A. Pedryc, R. Oláh

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

To raise the efficiency of plant regeneration we studied the important and necessary elements of the procedure. The embryogen capacity of 33 various grape genotypes were tested on four different induction media. We successfully obtained anther derived embryogenic calli in 27 genotypes with the range of 1-12%, this is the first reported protocol for embryogenesis from Korai bíbor, Odysseus, Orpheus and Pannon frankos cultivars. Two sorts of sterilization treatments were examined before inducing somatic embryos. For optimisation of grape regeneration system the propagation of calli was attempted in Richter 110 cultivar, there was no any significant differences in the measured values, but CPE medium proved more successful in maintaining embryogenic capacity of callus. We experienced high developmental differences between the propagated embryogenic culture of Richter 110, Teleki 5C and Chardonnay derived from MSNOA liquid medium and from MSE solid medium. Regenerated plants from embryogenic callus were obtained in 21 genotypes, in Chardonnay cultivar CP medium influenced more positively the plant regeneration than the MS/2 medium.

Original languageEnglish
Pages (from-to)139-149
Number of pages11
JournalActa Alimentaria
Volume40
DOIs
Publication statusPublished - Jan 1 2011

Fingerprint

wine quality
Vitis
Bony Callus
Wine
small fruits
Regeneration
grapes
Fruit
callus
Genotype
cultivars
genotype
somatic embryos
anthers
Embryonic Development
embryogenesis
Embryonic Structures
liquids

Keywords

  • 2,4-D (2,4-dichlorophenoxyacetic acid)
  • BAP (6-benzylaminopurine)
  • Embryogenic callus
  • NOA (2-naphthoxyacetic acid)
  • TDZ (thidiazuron)
  • Vitis

ASJC Scopus subject areas

  • Food Science

Cite this

Evaluation of regeneration capacity in grape towards the improvement of new cultivars with enhanced berry and wine quality. / Novák, E.; Zok, A.; Forgács, I.; Pedryc, A.; Oláh, R.

In: Acta Alimentaria, Vol. 40, 01.01.2011, p. 139-149.

Research output: Contribution to journalArticle

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