Evaluation of pseudorabies virus as a gene transfer vector and an oncolytic agent for human tumor cells

Z. Boldogkői, Andras Bratincsak, Istvan Fodor

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Background. Psuedorabies virus (PrV) is a neurotropic herpesvirus with a wide host - range including several mammalian species, but the virus is non - pathogenic for humans. We have evaluated PrV as a gene delivery vector for human tumor cells. Materials and Methods: SK-N-SH (neuroblastoma), U-87MG (glioblastoma) and Hep-2 (hepatoma) cells were infected with five different mutant PrV strains carrying the lacZ or gfp reporter gene. Mouse and human colon tumors were treated with a ribonucleotide reductase null mutant PrV in a mouse model. Results: PrV penetrated the cells and expressed the reporter genes. The viruses could replicate and egress mature virions from the infected cells. The tested virus could infect and destroy both mouse and human tumors in vivo without signs of disease related to PrV infection. Conclusion: PrV can infect and spread in human cell culture and in tumor tissues. Thus, PrV might be considered as a potential gene delivery vector to human tumor cells, as well as an oncolytic agent for human tumors.

Original languageEnglish
Pages (from-to)2153-2159
Number of pages7
JournalAnticancer Research
Volume22
Issue number4
Publication statusPublished - 2002

Fingerprint

Suid Herpesvirus 1
Viruses
Genes
Neoplasms
Reporter Genes
Ribonucleotide Reductases
Herpesviridae
Host Specificity
Virus Diseases
Glioblastoma
Neuroblastoma
Virion
Hepatocellular Carcinoma
Colon
Cell Culture Techniques

Keywords

  • Colon carcinoma
  • Gene therapy
  • Gene transfer
  • Herpesvirus
  • Pseudorabies virus
  • Tumor

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Evaluation of pseudorabies virus as a gene transfer vector and an oncolytic agent for human tumor cells. / Boldogkői, Z.; Bratincsak, Andras; Fodor, Istvan.

In: Anticancer Research, Vol. 22, No. 4, 2002, p. 2153-2159.

Research output: Contribution to journalArticle

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N2 - Background. Psuedorabies virus (PrV) is a neurotropic herpesvirus with a wide host - range including several mammalian species, but the virus is non - pathogenic for humans. We have evaluated PrV as a gene delivery vector for human tumor cells. Materials and Methods: SK-N-SH (neuroblastoma), U-87MG (glioblastoma) and Hep-2 (hepatoma) cells were infected with five different mutant PrV strains carrying the lacZ or gfp reporter gene. Mouse and human colon tumors were treated with a ribonucleotide reductase null mutant PrV in a mouse model. Results: PrV penetrated the cells and expressed the reporter genes. The viruses could replicate and egress mature virions from the infected cells. The tested virus could infect and destroy both mouse and human tumors in vivo without signs of disease related to PrV infection. Conclusion: PrV can infect and spread in human cell culture and in tumor tissues. Thus, PrV might be considered as a potential gene delivery vector to human tumor cells, as well as an oncolytic agent for human tumors.

AB - Background. Psuedorabies virus (PrV) is a neurotropic herpesvirus with a wide host - range including several mammalian species, but the virus is non - pathogenic for humans. We have evaluated PrV as a gene delivery vector for human tumor cells. Materials and Methods: SK-N-SH (neuroblastoma), U-87MG (glioblastoma) and Hep-2 (hepatoma) cells were infected with five different mutant PrV strains carrying the lacZ or gfp reporter gene. Mouse and human colon tumors were treated with a ribonucleotide reductase null mutant PrV in a mouse model. Results: PrV penetrated the cells and expressed the reporter genes. The viruses could replicate and egress mature virions from the infected cells. The tested virus could infect and destroy both mouse and human tumors in vivo without signs of disease related to PrV infection. Conclusion: PrV can infect and spread in human cell culture and in tumor tissues. Thus, PrV might be considered as a potential gene delivery vector to human tumor cells, as well as an oncolytic agent for human tumors.

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