Evaluation of pectin-reinforced supported liquid membranes containing carbonic anhydrase

The role of ionic liquid on enzyme stability and CO2 separation performance

N. Nemestóthy, Péter Bakonyi, Zsófia Németh, K. Bélafi-Bakó

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

In this paper, pectin-reinforced, supported liquid membranes (SLMs) prepared with carbonic anhydrase (CA) were investigated for CO2/N2 separation. In the first part of the study, the effect of [Bmim][NTf2] ionic liquid (IL) – as possible solvent to fill the pores of cellulose acetate support during SLM fabrication – on enzyme activity was tested. It turned out that this particular IL caused rapid and severe loss of initial biocatalyst activity, which fact can be seen as a threat in the membrane process design. Afterwards, the stability of pectin-containing SLMs (containing CA but lacking the IL having adverse impact) was addressed and their improved resistance against higher transmembrane pressures (up to 7.2 bar) was found, representing an approx. 3-fold enhancement compared to their control. Thereafter, the performance of the membranes was tested under single and mixed gas conditions with carbon dioxide and nitrogen. Employing single gases, it was demonstrated that CA enzyme could notably increase CO2 permeability (from 55 to 93 Barrer), while that of N2 remained unchanged (1.6-1.7 Barrer). Thus, the highest CO2/N2 theoretical selectivity was attained as 54 using the pectin-reinforced SLMs enriched with CA biocatalyst. For comparison, the outcomes were plotted on the Robeson upper-bound.

Original languageEnglish
Pages (from-to)59-63
Number of pages5
JournalJournal of CO2 Utilization
Volume24
DOIs
Publication statusPublished - Mar 1 2018

Fingerprint

Carbonic anhydrase
Ionic Liquids
Liquid membranes
Carbonic Anhydrases
Ionic liquids
Enzymes
enzyme
membrane
liquid
Biocatalysts
Gases
Membranes
Enzyme activity
Carbon Dioxide
Cellulose
Process design
Carbon dioxide
Nitrogen
gas
Fabrication

Keywords

  • Carbonic anhydrase
  • CO separation
  • Gas separation
  • Ionic liquid
  • Supported liquid membrane

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Waste Management and Disposal
  • Process Chemistry and Technology

Cite this

@article{e7bc2f1455e542e9b1039c5bbbe002b9,
title = "Evaluation of pectin-reinforced supported liquid membranes containing carbonic anhydrase: The role of ionic liquid on enzyme stability and CO2 separation performance",
abstract = "In this paper, pectin-reinforced, supported liquid membranes (SLMs) prepared with carbonic anhydrase (CA) were investigated for CO2/N2 separation. In the first part of the study, the effect of [Bmim][NTf2] ionic liquid (IL) – as possible solvent to fill the pores of cellulose acetate support during SLM fabrication – on enzyme activity was tested. It turned out that this particular IL caused rapid and severe loss of initial biocatalyst activity, which fact can be seen as a threat in the membrane process design. Afterwards, the stability of pectin-containing SLMs (containing CA but lacking the IL having adverse impact) was addressed and their improved resistance against higher transmembrane pressures (up to 7.2 bar) was found, representing an approx. 3-fold enhancement compared to their control. Thereafter, the performance of the membranes was tested under single and mixed gas conditions with carbon dioxide and nitrogen. Employing single gases, it was demonstrated that CA enzyme could notably increase CO2 permeability (from 55 to 93 Barrer), while that of N2 remained unchanged (1.6-1.7 Barrer). Thus, the highest CO2/N2 theoretical selectivity was attained as 54 using the pectin-reinforced SLMs enriched with CA biocatalyst. For comparison, the outcomes were plotted on the Robeson upper-bound.",
keywords = "Carbonic anhydrase, CO separation, Gas separation, Ionic liquid, Supported liquid membrane",
author = "N. Nemest{\'o}thy and P{\'e}ter Bakonyi and Zs{\'o}fia N{\'e}meth and K. B{\'e}lafi-Bak{\'o}",
year = "2018",
month = "3",
day = "1",
doi = "10.1016/j.jcou.2017.12.001",
language = "English",
volume = "24",
pages = "59--63",
journal = "Journal of CO2 Utilization",
issn = "2212-9820",
publisher = "Elsevier BV",

}

TY - JOUR

T1 - Evaluation of pectin-reinforced supported liquid membranes containing carbonic anhydrase

T2 - The role of ionic liquid on enzyme stability and CO2 separation performance

AU - Nemestóthy, N.

AU - Bakonyi, Péter

AU - Németh, Zsófia

AU - Bélafi-Bakó, K.

PY - 2018/3/1

Y1 - 2018/3/1

N2 - In this paper, pectin-reinforced, supported liquid membranes (SLMs) prepared with carbonic anhydrase (CA) were investigated for CO2/N2 separation. In the first part of the study, the effect of [Bmim][NTf2] ionic liquid (IL) – as possible solvent to fill the pores of cellulose acetate support during SLM fabrication – on enzyme activity was tested. It turned out that this particular IL caused rapid and severe loss of initial biocatalyst activity, which fact can be seen as a threat in the membrane process design. Afterwards, the stability of pectin-containing SLMs (containing CA but lacking the IL having adverse impact) was addressed and their improved resistance against higher transmembrane pressures (up to 7.2 bar) was found, representing an approx. 3-fold enhancement compared to their control. Thereafter, the performance of the membranes was tested under single and mixed gas conditions with carbon dioxide and nitrogen. Employing single gases, it was demonstrated that CA enzyme could notably increase CO2 permeability (from 55 to 93 Barrer), while that of N2 remained unchanged (1.6-1.7 Barrer). Thus, the highest CO2/N2 theoretical selectivity was attained as 54 using the pectin-reinforced SLMs enriched with CA biocatalyst. For comparison, the outcomes were plotted on the Robeson upper-bound.

AB - In this paper, pectin-reinforced, supported liquid membranes (SLMs) prepared with carbonic anhydrase (CA) were investigated for CO2/N2 separation. In the first part of the study, the effect of [Bmim][NTf2] ionic liquid (IL) – as possible solvent to fill the pores of cellulose acetate support during SLM fabrication – on enzyme activity was tested. It turned out that this particular IL caused rapid and severe loss of initial biocatalyst activity, which fact can be seen as a threat in the membrane process design. Afterwards, the stability of pectin-containing SLMs (containing CA but lacking the IL having adverse impact) was addressed and their improved resistance against higher transmembrane pressures (up to 7.2 bar) was found, representing an approx. 3-fold enhancement compared to their control. Thereafter, the performance of the membranes was tested under single and mixed gas conditions with carbon dioxide and nitrogen. Employing single gases, it was demonstrated that CA enzyme could notably increase CO2 permeability (from 55 to 93 Barrer), while that of N2 remained unchanged (1.6-1.7 Barrer). Thus, the highest CO2/N2 theoretical selectivity was attained as 54 using the pectin-reinforced SLMs enriched with CA biocatalyst. For comparison, the outcomes were plotted on the Robeson upper-bound.

KW - Carbonic anhydrase

KW - CO separation

KW - Gas separation

KW - Ionic liquid

KW - Supported liquid membrane

UR - http://www.scopus.com/inward/record.url?scp=85037689530&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85037689530&partnerID=8YFLogxK

U2 - 10.1016/j.jcou.2017.12.001

DO - 10.1016/j.jcou.2017.12.001

M3 - Article

VL - 24

SP - 59

EP - 63

JO - Journal of CO2 Utilization

JF - Journal of CO2 Utilization

SN - 2212-9820

ER -