Evaluation of oxidative stress in the thrombolysis of pulmonary embolism

Diana Mühl, Réka Füredi, Julia Cristofari, Subhamay Ghosh, L. Bogár, Balázs Borsiczki, B. Gasz, E. Rőth, J. Lantos

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Background: To analyse leukocyte function parameters and oxidative stress (OS) in patients with acute pulmonary embolism (PE) treated with thrombolytics. Methods: Fifteen patients undergoing thrombolysis (TL) with ultra-high dose streptokinase (n = 8), or alteplase (tPA) (n = 7) treatment were studied. Blood samples were collected prior to TL, and then 8 h, 1, 3, 5 and 30 days after treatment. Malondialdehyde (MDA), reduced glutathione (GSH), plasma protein sulfhydryl groups (PSH) levels, superoxide dismutase (SOD) and myeloperoxidase enzyme (MPO) activities were measured in plasma or whole blood for monitoring of the OS markers. Production of reactive oxygen species (ROS) in whole blood was measured by luminol dependent chemiluminescence. Flow cytometry was used to determine CD11a, CD18, and CD97 surface antigen expression on leukocytes. Results: The elevated MDA, ROS and MPO, decreased GSH and PSH levels indicated the presence of OS in patients with PE. MDA significantly (P <0.05) increased, GSH significantly (P <0.05) decreased following thrombolysis. ROS production peaked on the 3rd and 5th days. TL was accompanied by significant decrease in granulocyte and monocyte CD11a and CD18 as well as in granulocyte CD97 expression (P <0.05). Conclusion: PE led to OS that was augmented following TL. Decreased adhesion molecule expression of circulating leukocytes in the early phase of TL reflects the pathological leukocyte endothelial cell interactions.

Original languageEnglish
Pages (from-to)221-228
Number of pages8
JournalJournal of Thrombosis and Thrombolysis
Volume22
Issue number3
DOIs
Publication statusPublished - Dec 2006

Fingerprint

Pulmonary Embolism
Oxidative Stress
Leukocytes
Malondialdehyde
Reactive Oxygen Species
Granulocytes
Peroxidase
Luminol
Streptokinase
Tissue Plasminogen Activator
Enzymes
Surface Antigens
Luminescence
Cell Communication
Superoxide Dismutase
Glutathione
Blood Proteins
Monocytes
Flow Cytometry
Endothelial Cells

Keywords

  • Leukocyte activation
  • Oxidative stress
  • Pulmonary embolism
  • Thrombolysis

ASJC Scopus subject areas

  • Hematology
  • Cardiology and Cardiovascular Medicine

Cite this

Evaluation of oxidative stress in the thrombolysis of pulmonary embolism. / Mühl, Diana; Füredi, Réka; Cristofari, Julia; Ghosh, Subhamay; Bogár, L.; Borsiczki, Balázs; Gasz, B.; Rőth, E.; Lantos, J.

In: Journal of Thrombosis and Thrombolysis, Vol. 22, No. 3, 12.2006, p. 221-228.

Research output: Contribution to journalArticle

Mühl, Diana ; Füredi, Réka ; Cristofari, Julia ; Ghosh, Subhamay ; Bogár, L. ; Borsiczki, Balázs ; Gasz, B. ; Rőth, E. ; Lantos, J. / Evaluation of oxidative stress in the thrombolysis of pulmonary embolism. In: Journal of Thrombosis and Thrombolysis. 2006 ; Vol. 22, No. 3. pp. 221-228.
@article{d45cfce032234733a539741684c7766e,
title = "Evaluation of oxidative stress in the thrombolysis of pulmonary embolism",
abstract = "Background: To analyse leukocyte function parameters and oxidative stress (OS) in patients with acute pulmonary embolism (PE) treated with thrombolytics. Methods: Fifteen patients undergoing thrombolysis (TL) with ultra-high dose streptokinase (n = 8), or alteplase (tPA) (n = 7) treatment were studied. Blood samples were collected prior to TL, and then 8 h, 1, 3, 5 and 30 days after treatment. Malondialdehyde (MDA), reduced glutathione (GSH), plasma protein sulfhydryl groups (PSH) levels, superoxide dismutase (SOD) and myeloperoxidase enzyme (MPO) activities were measured in plasma or whole blood for monitoring of the OS markers. Production of reactive oxygen species (ROS) in whole blood was measured by luminol dependent chemiluminescence. Flow cytometry was used to determine CD11a, CD18, and CD97 surface antigen expression on leukocytes. Results: The elevated MDA, ROS and MPO, decreased GSH and PSH levels indicated the presence of OS in patients with PE. MDA significantly (P <0.05) increased, GSH significantly (P <0.05) decreased following thrombolysis. ROS production peaked on the 3rd and 5th days. TL was accompanied by significant decrease in granulocyte and monocyte CD11a and CD18 as well as in granulocyte CD97 expression (P <0.05). Conclusion: PE led to OS that was augmented following TL. Decreased adhesion molecule expression of circulating leukocytes in the early phase of TL reflects the pathological leukocyte endothelial cell interactions.",
keywords = "Leukocyte activation, Oxidative stress, Pulmonary embolism, Thrombolysis",
author = "Diana M{\"u}hl and R{\'e}ka F{\"u}redi and Julia Cristofari and Subhamay Ghosh and L. Bog{\'a}r and Bal{\'a}zs Borsiczki and B. Gasz and E. Rőth and J. Lantos",
year = "2006",
month = "12",
doi = "10.1007/s11239-006-9035-2",
language = "English",
volume = "22",
pages = "221--228",
journal = "Journal of Thrombosis and Thrombolysis",
issn = "0929-5305",
publisher = "Springer Netherlands",
number = "3",

}

TY - JOUR

T1 - Evaluation of oxidative stress in the thrombolysis of pulmonary embolism

AU - Mühl, Diana

AU - Füredi, Réka

AU - Cristofari, Julia

AU - Ghosh, Subhamay

AU - Bogár, L.

AU - Borsiczki, Balázs

AU - Gasz, B.

AU - Rőth, E.

AU - Lantos, J.

PY - 2006/12

Y1 - 2006/12

N2 - Background: To analyse leukocyte function parameters and oxidative stress (OS) in patients with acute pulmonary embolism (PE) treated with thrombolytics. Methods: Fifteen patients undergoing thrombolysis (TL) with ultra-high dose streptokinase (n = 8), or alteplase (tPA) (n = 7) treatment were studied. Blood samples were collected prior to TL, and then 8 h, 1, 3, 5 and 30 days after treatment. Malondialdehyde (MDA), reduced glutathione (GSH), plasma protein sulfhydryl groups (PSH) levels, superoxide dismutase (SOD) and myeloperoxidase enzyme (MPO) activities were measured in plasma or whole blood for monitoring of the OS markers. Production of reactive oxygen species (ROS) in whole blood was measured by luminol dependent chemiluminescence. Flow cytometry was used to determine CD11a, CD18, and CD97 surface antigen expression on leukocytes. Results: The elevated MDA, ROS and MPO, decreased GSH and PSH levels indicated the presence of OS in patients with PE. MDA significantly (P <0.05) increased, GSH significantly (P <0.05) decreased following thrombolysis. ROS production peaked on the 3rd and 5th days. TL was accompanied by significant decrease in granulocyte and monocyte CD11a and CD18 as well as in granulocyte CD97 expression (P <0.05). Conclusion: PE led to OS that was augmented following TL. Decreased adhesion molecule expression of circulating leukocytes in the early phase of TL reflects the pathological leukocyte endothelial cell interactions.

AB - Background: To analyse leukocyte function parameters and oxidative stress (OS) in patients with acute pulmonary embolism (PE) treated with thrombolytics. Methods: Fifteen patients undergoing thrombolysis (TL) with ultra-high dose streptokinase (n = 8), or alteplase (tPA) (n = 7) treatment were studied. Blood samples were collected prior to TL, and then 8 h, 1, 3, 5 and 30 days after treatment. Malondialdehyde (MDA), reduced glutathione (GSH), plasma protein sulfhydryl groups (PSH) levels, superoxide dismutase (SOD) and myeloperoxidase enzyme (MPO) activities were measured in plasma or whole blood for monitoring of the OS markers. Production of reactive oxygen species (ROS) in whole blood was measured by luminol dependent chemiluminescence. Flow cytometry was used to determine CD11a, CD18, and CD97 surface antigen expression on leukocytes. Results: The elevated MDA, ROS and MPO, decreased GSH and PSH levels indicated the presence of OS in patients with PE. MDA significantly (P <0.05) increased, GSH significantly (P <0.05) decreased following thrombolysis. ROS production peaked on the 3rd and 5th days. TL was accompanied by significant decrease in granulocyte and monocyte CD11a and CD18 as well as in granulocyte CD97 expression (P <0.05). Conclusion: PE led to OS that was augmented following TL. Decreased adhesion molecule expression of circulating leukocytes in the early phase of TL reflects the pathological leukocyte endothelial cell interactions.

KW - Leukocyte activation

KW - Oxidative stress

KW - Pulmonary embolism

KW - Thrombolysis

UR - http://www.scopus.com/inward/record.url?scp=33845259832&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33845259832&partnerID=8YFLogxK

U2 - 10.1007/s11239-006-9035-2

DO - 10.1007/s11239-006-9035-2

M3 - Article

VL - 22

SP - 221

EP - 228

JO - Journal of Thrombosis and Thrombolysis

JF - Journal of Thrombosis and Thrombolysis

SN - 0929-5305

IS - 3

ER -