Evaluation of monoclonal antibodies having specificity for human IgG sub-classes: Results of an IUIS/WHO collaborative study

R. Jefferis, C. B. Reimer, F. Skvaril, G. de Lange, N. R. Ling, J. Lowe, M. R. Walker, D. J. Phillips, C. H. Aloisio, T. W. Wells, J. P. Vaerman, C. G. Magnusson, H. Kubagawa, M. Cooper, F. Vartdal, B. Vandvik, J. J. Haaijman, O. Makela, A. Sarnesto, Z. LandoJ. Gergely, E. Rajnavolgyi, G. László, J. Radl, G. A. Molinaro

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Abstract

Seventy-four monoclonal antibodies (McAb) of putative specificity for human IgG (11), the IgG subclasses (59) or Gm allotypes (4) have been evaluated for reactivity and specificity in eight laboratories employing different assay techniques or protocols. For the IgG, IgG3, IgG4, G1m(f) and G3m(u) specificities McAb have been produced that can be satisfactorily applied in most methodologies employed and have potential as reference reagents. The IgG1 and particularly IgG2 specificities proved problematical with all McAb evaluated demonstrating apparent assay restriction and whilst performing well in some assays proved to be poor or inactive reagents in others. However, the study identifies McAb individually suited to application within most commonly employed methodologies. Epitope display is the probable variability rather than capricious behaviour by the McAb. IgG1 and IgG2 were the least immunogenic of the sub-class proteins and there is evidence that epitope display is influenced by the physical and chemical procedures used to immobilize or fix antigen - a common requirement in the assay systems studied.

Original languageEnglish
Pages (from-to)223-252
Number of pages30
JournalImmunology Letters
Volume10
Issue number3-4
DOIs
Publication statusPublished - 1985

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Antibody Specificity
Immunoglobulin G
Monoclonal Antibodies
Epitopes
Immunoglobulin Gm Allotypes
Antigens

Keywords

  • human
  • IgG
  • monoclonal antibodies

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Jefferis, R., Reimer, C. B., Skvaril, F., de Lange, G., Ling, N. R., Lowe, J., ... Molinaro, G. A. (1985). Evaluation of monoclonal antibodies having specificity for human IgG sub-classes: Results of an IUIS/WHO collaborative study. Immunology Letters, 10(3-4), 223-252. https://doi.org/10.1016/0165-2478(85)90082-3

Evaluation of monoclonal antibodies having specificity for human IgG sub-classes : Results of an IUIS/WHO collaborative study. / Jefferis, R.; Reimer, C. B.; Skvaril, F.; de Lange, G.; Ling, N. R.; Lowe, J.; Walker, M. R.; Phillips, D. J.; Aloisio, C. H.; Wells, T. W.; Vaerman, J. P.; Magnusson, C. G.; Kubagawa, H.; Cooper, M.; Vartdal, F.; Vandvik, B.; Haaijman, J. J.; Makela, O.; Sarnesto, A.; Lando, Z.; Gergely, J.; Rajnavolgyi, E.; László, G.; Radl, J.; Molinaro, G. A.

In: Immunology Letters, Vol. 10, No. 3-4, 1985, p. 223-252.

Research output: Contribution to journalArticle

Jefferis, R, Reimer, CB, Skvaril, F, de Lange, G, Ling, NR, Lowe, J, Walker, MR, Phillips, DJ, Aloisio, CH, Wells, TW, Vaerman, JP, Magnusson, CG, Kubagawa, H, Cooper, M, Vartdal, F, Vandvik, B, Haaijman, JJ, Makela, O, Sarnesto, A, Lando, Z, Gergely, J, Rajnavolgyi, E, László, G, Radl, J & Molinaro, GA 1985, 'Evaluation of monoclonal antibodies having specificity for human IgG sub-classes: Results of an IUIS/WHO collaborative study', Immunology Letters, vol. 10, no. 3-4, pp. 223-252. https://doi.org/10.1016/0165-2478(85)90082-3
Jefferis, R. ; Reimer, C. B. ; Skvaril, F. ; de Lange, G. ; Ling, N. R. ; Lowe, J. ; Walker, M. R. ; Phillips, D. J. ; Aloisio, C. H. ; Wells, T. W. ; Vaerman, J. P. ; Magnusson, C. G. ; Kubagawa, H. ; Cooper, M. ; Vartdal, F. ; Vandvik, B. ; Haaijman, J. J. ; Makela, O. ; Sarnesto, A. ; Lando, Z. ; Gergely, J. ; Rajnavolgyi, E. ; László, G. ; Radl, J. ; Molinaro, G. A. / Evaluation of monoclonal antibodies having specificity for human IgG sub-classes : Results of an IUIS/WHO collaborative study. In: Immunology Letters. 1985 ; Vol. 10, No. 3-4. pp. 223-252.
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AU - Gergely, J.

AU - Rajnavolgyi, E.

AU - László, G.

AU - Radl, J.

AU - Molinaro, G. A.

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