Evaluation of laser-speckle contrast image analysis techniques in the cortical microcirculation of piglets

F. Domoki, Dániel Zölei, Orsolya Oláh, Valéria Tóth-Szuki, B. Hopp, F. Bari, T. Smausz

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

A new laser speckle-contrast analysis (LASCA) technique based on multi-exposure imaging was employed to simultaneously study pial arteriolar responses with cerebrocortical perfusion changes to various vasodilator (5-10% CO 2 ventilation, bradykinin (1-10μM), N-methyl-d-aspartate (100μM)) vasoconstrictor (10-100μM noradrenaline, 1M KCl), or neutral (2.1% H 2 ventilation) stimuli as well as to asphyxia in the newborn piglet. Anesthetized, ventilated animals (n=20) were fitted with closed cranial windows. Multiple exposure laser-speckle image series (1-100ms) were obtained using a near infrared diode laser (λ=808nm). The autocorrelation decay time (τ) of speckle fluctuations was determined over pial arterioles and parenchymal areas to express 1/τ being proportional to blood flow velocity by two different LASCA techniques: our novel multi-exposure or a single exposure (2 and 20ms) approach. 1/τ values yielded by different LASCA techniques were not significantly different at most points. LASCA easily detected both increases and decreases in cortical blood flow (CoBF). Cortical 1/τ changes to hypercapnia closely matched quantitative CoBF data determined previously, and were also in accordance with increases of pial arteriolar blood flow, calculated from arteriolar flow velocity and cross sectional area changes. In summary, LASCA emerges as an appealing method to simultaneously study microvascular reactivity and cortical perfusion changes in the piglet.

Original languageEnglish
Pages (from-to)311-317
Number of pages7
JournalMicrovascular Research
Volume83
Issue number3
DOIs
Publication statusPublished - May 2012

Fingerprint

Microcirculation
Speckle
Image analysis
Lasers
Blood
Ventilation
Flow velocity
Perfusion
Semiconductor Lasers
Blood Flow Velocity
Hypercapnia
Asphyxia
Arterioles
Bradykinin
Vasoconstrictor Agents
Carbon Monoxide
Vasodilator Agents
Aspartic Acid
Autocorrelation
Norepinephrine

ASJC Scopus subject areas

  • Biochemistry
  • Cardiology and Cardiovascular Medicine
  • Cell Biology

Cite this

Evaluation of laser-speckle contrast image analysis techniques in the cortical microcirculation of piglets. / Domoki, F.; Zölei, Dániel; Oláh, Orsolya; Tóth-Szuki, Valéria; Hopp, B.; Bari, F.; Smausz, T.

In: Microvascular Research, Vol. 83, No. 3, 05.2012, p. 311-317.

Research output: Contribution to journalArticle

@article{2a775151fdb248bc8b221fb85da5ada7,
title = "Evaluation of laser-speckle contrast image analysis techniques in the cortical microcirculation of piglets",
abstract = "A new laser speckle-contrast analysis (LASCA) technique based on multi-exposure imaging was employed to simultaneously study pial arteriolar responses with cerebrocortical perfusion changes to various vasodilator (5-10{\%} CO 2 ventilation, bradykinin (1-10μM), N-methyl-d-aspartate (100μM)) vasoconstrictor (10-100μM noradrenaline, 1M KCl), or neutral (2.1{\%} H 2 ventilation) stimuli as well as to asphyxia in the newborn piglet. Anesthetized, ventilated animals (n=20) were fitted with closed cranial windows. Multiple exposure laser-speckle image series (1-100ms) were obtained using a near infrared diode laser (λ=808nm). The autocorrelation decay time (τ) of speckle fluctuations was determined over pial arterioles and parenchymal areas to express 1/τ being proportional to blood flow velocity by two different LASCA techniques: our novel multi-exposure or a single exposure (2 and 20ms) approach. 1/τ values yielded by different LASCA techniques were not significantly different at most points. LASCA easily detected both increases and decreases in cortical blood flow (CoBF). Cortical 1/τ changes to hypercapnia closely matched quantitative CoBF data determined previously, and were also in accordance with increases of pial arteriolar blood flow, calculated from arteriolar flow velocity and cross sectional area changes. In summary, LASCA emerges as an appealing method to simultaneously study microvascular reactivity and cortical perfusion changes in the piglet.",
author = "F. Domoki and D{\'a}niel Z{\"o}lei and Orsolya Ol{\'a}h and Val{\'e}ria T{\'o}th-Szuki and B. Hopp and F. Bari and T. Smausz",
year = "2012",
month = "5",
doi = "10.1016/j.mvr.2012.01.003",
language = "English",
volume = "83",
pages = "311--317",
journal = "Microvascular Research",
issn = "0026-2862",
publisher = "Academic Press Inc.",
number = "3",

}

TY - JOUR

T1 - Evaluation of laser-speckle contrast image analysis techniques in the cortical microcirculation of piglets

AU - Domoki, F.

AU - Zölei, Dániel

AU - Oláh, Orsolya

AU - Tóth-Szuki, Valéria

AU - Hopp, B.

AU - Bari, F.

AU - Smausz, T.

PY - 2012/5

Y1 - 2012/5

N2 - A new laser speckle-contrast analysis (LASCA) technique based on multi-exposure imaging was employed to simultaneously study pial arteriolar responses with cerebrocortical perfusion changes to various vasodilator (5-10% CO 2 ventilation, bradykinin (1-10μM), N-methyl-d-aspartate (100μM)) vasoconstrictor (10-100μM noradrenaline, 1M KCl), or neutral (2.1% H 2 ventilation) stimuli as well as to asphyxia in the newborn piglet. Anesthetized, ventilated animals (n=20) were fitted with closed cranial windows. Multiple exposure laser-speckle image series (1-100ms) were obtained using a near infrared diode laser (λ=808nm). The autocorrelation decay time (τ) of speckle fluctuations was determined over pial arterioles and parenchymal areas to express 1/τ being proportional to blood flow velocity by two different LASCA techniques: our novel multi-exposure or a single exposure (2 and 20ms) approach. 1/τ values yielded by different LASCA techniques were not significantly different at most points. LASCA easily detected both increases and decreases in cortical blood flow (CoBF). Cortical 1/τ changes to hypercapnia closely matched quantitative CoBF data determined previously, and were also in accordance with increases of pial arteriolar blood flow, calculated from arteriolar flow velocity and cross sectional area changes. In summary, LASCA emerges as an appealing method to simultaneously study microvascular reactivity and cortical perfusion changes in the piglet.

AB - A new laser speckle-contrast analysis (LASCA) technique based on multi-exposure imaging was employed to simultaneously study pial arteriolar responses with cerebrocortical perfusion changes to various vasodilator (5-10% CO 2 ventilation, bradykinin (1-10μM), N-methyl-d-aspartate (100μM)) vasoconstrictor (10-100μM noradrenaline, 1M KCl), or neutral (2.1% H 2 ventilation) stimuli as well as to asphyxia in the newborn piglet. Anesthetized, ventilated animals (n=20) were fitted with closed cranial windows. Multiple exposure laser-speckle image series (1-100ms) were obtained using a near infrared diode laser (λ=808nm). The autocorrelation decay time (τ) of speckle fluctuations was determined over pial arterioles and parenchymal areas to express 1/τ being proportional to blood flow velocity by two different LASCA techniques: our novel multi-exposure or a single exposure (2 and 20ms) approach. 1/τ values yielded by different LASCA techniques were not significantly different at most points. LASCA easily detected both increases and decreases in cortical blood flow (CoBF). Cortical 1/τ changes to hypercapnia closely matched quantitative CoBF data determined previously, and were also in accordance with increases of pial arteriolar blood flow, calculated from arteriolar flow velocity and cross sectional area changes. In summary, LASCA emerges as an appealing method to simultaneously study microvascular reactivity and cortical perfusion changes in the piglet.

UR - http://www.scopus.com/inward/record.url?scp=84859506544&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84859506544&partnerID=8YFLogxK

U2 - 10.1016/j.mvr.2012.01.003

DO - 10.1016/j.mvr.2012.01.003

M3 - Article

C2 - 22306444

AN - SCOPUS:84859506544

VL - 83

SP - 311

EP - 317

JO - Microvascular Research

JF - Microvascular Research

SN - 0026-2862

IS - 3

ER -