Evaluation of goose serum amyloid a acute phase response by enzyme-linked immunosorbent assay

Beáta Marianna Kovács, Mathilda J M Toussaint, E. Gruys, Ibolya B. Fábián, L. Szilágyi, J. Janan, P. Rudas

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Serum amyloid A (SAA) is of interest as the circulating precursor of amyloid A protein, the fibrillar component of AA (secondary) amyloid deposits, and also as an extremely sensitive and rapid major acute phase protein. Serum concentrations of acute phase proteins (APPs) provide valuable information about the diagnosis and prognosis of various diseases, and thus the relevance of APPs for monitoring the health status of domestic animals is widely accepted. More importantly, the measurement of SAA concentration assists in assessing the prognosis in secondary amyloidosis, which is a common disease of geese, affecting an increasing number of animals. In the present study we introduce a highly sensitive goose-specific ELISA method for measuring SAA concentration in goose serum or plasma samples. Samples were taken from geese of the Landes Grey and Hungarian White breeds, which were stimulated for an acute phase reaction by administration of a commercially available fowl cholera vaccine containing inactivated Pasteurella multocida. Strong and characteristically rapid acute phase responses were measured in both breeds, peaking at approximately 24 h after inoculation. The maximum SAA concentration was 1200 μg/ml. At 72 h post-inoculation, the concentrations returned to pre-inoculation values. There was significantly (p = 0.004) less intense response in the control groups; however, a very mild increase of SAA levels was detected due to the stress inevitably caused by the sampling procedure.

Original languageEnglish
Pages (from-to)349-357
Number of pages9
JournalActa Veterinaria Hungarica
Volume55
Issue number3
DOIs
Publication statusPublished - 2007

Fingerprint

Geese
Serum Amyloid A Protein
Acute-Phase Reaction
amyloid
geese
Amyloid
Enzyme-Linked Immunosorbent Assay
enzyme-linked immunosorbent assay
Acute-Phase Proteins
acute phase proteins
Serum
vaccination
prognosis
Cholera Vaccines
Pasteurella multocida
fowl cholera
breeds
Domestic Animals
Amyloid Plaques
amyloidosis

Keywords

  • Acute phase response
  • Enzyme-linked immunosorbent assay
  • Goose
  • Serum amyloid A
  • Waterfowl

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Kovács, B. M., Toussaint, M. J. M., Gruys, E., Fábián, I. B., Szilágyi, L., Janan, J., & Rudas, P. (2007). Evaluation of goose serum amyloid a acute phase response by enzyme-linked immunosorbent assay. Acta Veterinaria Hungarica, 55(3), 349-357. https://doi.org/10.1556/AVet.55.2007.3.9

Evaluation of goose serum amyloid a acute phase response by enzyme-linked immunosorbent assay. / Kovács, Beáta Marianna; Toussaint, Mathilda J M; Gruys, E.; Fábián, Ibolya B.; Szilágyi, L.; Janan, J.; Rudas, P.

In: Acta Veterinaria Hungarica, Vol. 55, No. 3, 2007, p. 349-357.

Research output: Contribution to journalArticle

Kovács, BM, Toussaint, MJM, Gruys, E, Fábián, IB, Szilágyi, L, Janan, J & Rudas, P 2007, 'Evaluation of goose serum amyloid a acute phase response by enzyme-linked immunosorbent assay', Acta Veterinaria Hungarica, vol. 55, no. 3, pp. 349-357. https://doi.org/10.1556/AVet.55.2007.3.9
Kovács, Beáta Marianna ; Toussaint, Mathilda J M ; Gruys, E. ; Fábián, Ibolya B. ; Szilágyi, L. ; Janan, J. ; Rudas, P. / Evaluation of goose serum amyloid a acute phase response by enzyme-linked immunosorbent assay. In: Acta Veterinaria Hungarica. 2007 ; Vol. 55, No. 3. pp. 349-357.
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