The frost hardiness of 102 traditional and newly bred Hungarian Vitis vinifera and interspecific cultivars were tested using artificial frost treatments in climatic chambers. The sugar content of treated buds was analysed for sucrose, glucose, fructose and raffinose by over pressure layer chromatography. The traditional Hungarian cultivars belonging to convar. Pontica, are regarded as tender cultivars, but some of these varieties which can only be found in gene banks showed fairly good frost tolerance. In the new V. vinifera varieties, 'Cserszegi fuszeres' and 'Generosa' were the most hardy. Among the interspecific cultivars '2426' (Franco- American origin), 'Amadeus', 'Orpheus', 'Odysseus' and 'Pannon frankos' (V. amurensis origin) showed excellent frost resistance.