Evaluation of faecal flotation methods followed by species-specific PCR for detection of Echinococcus multilocularis in the definitive hosts

Z. Széll, Zsuzsanna Sréter-Lancz, T. Sréter

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Echinococcus multilocularis is one of the most pathogenic zoonotic parasites in the temperate and arctic region of the Northern Hemisphere. For estimating the potential risk of human infection in endemic areas, reliable antemortem methods are needed to detect the parasite in carnivore definitive hosts. The sensitivity of routine flotation techniques for detection of E. multilocularis eggs was found to be low (3-33%) depending on the flotation solution used (specific gravities = 1.3-1.4). An improved faecal flotation followed by a species-specific PCR is described with a sensitivity of 74% (95% CI = 62-84%) and a specificity of 100% (95% CI = 94-100%). These parameters are similar to those of the intestinal scraping technique (sensitivity = 78%, specificity = 100%). The sensitivity of the improved flotation was significantly higher (P <0.0001) than that of routine flotation techniques. The costs of the method are similar or lower than those of other antemortem diagnostic methods. Based on these data, the method is suitable for surveys of domesticated and wild carnivores.

Original languageEnglish
Pages (from-to)331-336
Number of pages6
JournalActa Parasitologica
Volume59
Issue number2
DOIs
Publication statusPublished - 2014

Fingerprint

Echinococcus multilocularis
Polymerase Chain Reaction
Parasites
Arctic Regions
Specific Gravity
Zoonoses
Eggs
Costs and Cost Analysis
Sensitivity and Specificity
Infection

Keywords

  • carnivores
  • definitive host
  • detection
  • Echinococcus multilocularis
  • eggs
  • flotation
  • PCR

ASJC Scopus subject areas

  • Parasitology
  • Medicine(all)

Cite this

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title = "Evaluation of faecal flotation methods followed by species-specific PCR for detection of Echinococcus multilocularis in the definitive hosts",
abstract = "Echinococcus multilocularis is one of the most pathogenic zoonotic parasites in the temperate and arctic region of the Northern Hemisphere. For estimating the potential risk of human infection in endemic areas, reliable antemortem methods are needed to detect the parasite in carnivore definitive hosts. The sensitivity of routine flotation techniques for detection of E. multilocularis eggs was found to be low (3-33{\%}) depending on the flotation solution used (specific gravities = 1.3-1.4). An improved faecal flotation followed by a species-specific PCR is described with a sensitivity of 74{\%} (95{\%} CI = 62-84{\%}) and a specificity of 100{\%} (95{\%} CI = 94-100{\%}). These parameters are similar to those of the intestinal scraping technique (sensitivity = 78{\%}, specificity = 100{\%}). The sensitivity of the improved flotation was significantly higher (P <0.0001) than that of routine flotation techniques. The costs of the method are similar or lower than those of other antemortem diagnostic methods. Based on these data, the method is suitable for surveys of domesticated and wild carnivores.",
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author = "Z. Sz{\'e}ll and Zsuzsanna Sr{\'e}ter-Lancz and T. Sr{\'e}ter",
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AU - Sréter, T.

PY - 2014

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AB - Echinococcus multilocularis is one of the most pathogenic zoonotic parasites in the temperate and arctic region of the Northern Hemisphere. For estimating the potential risk of human infection in endemic areas, reliable antemortem methods are needed to detect the parasite in carnivore definitive hosts. The sensitivity of routine flotation techniques for detection of E. multilocularis eggs was found to be low (3-33%) depending on the flotation solution used (specific gravities = 1.3-1.4). An improved faecal flotation followed by a species-specific PCR is described with a sensitivity of 74% (95% CI = 62-84%) and a specificity of 100% (95% CI = 94-100%). These parameters are similar to those of the intestinal scraping technique (sensitivity = 78%, specificity = 100%). The sensitivity of the improved flotation was significantly higher (P <0.0001) than that of routine flotation techniques. The costs of the method are similar or lower than those of other antemortem diagnostic methods. Based on these data, the method is suitable for surveys of domesticated and wild carnivores.

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