Evaluation of efficacy of entomopathogenic nematodes against larvae of Lucilia sericata (Meigen, 1826) (Diptera: Calliphoridae)

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Abstract

The blowfly Lucilia sericata (Meigen, 1826) (Diptera: Calliphoridae) is the primary agent of cutaneous myiasis of sheep in northern Europe, southern Africa, Australia and New Zealand. As the application of chemicals has several disadvantages, alternative control measures of traumatic myiasis of livestock must be developed. In this study, the use of entomopathogenic nematodes (EPNs) as potential biocontrol agents against second instar larvae of Lucilia sericata was considered. The following nematode species were tested: Heterorhabditis bacteriophora (IS 5, HHU 1, Hmo1, HNC 1, HAZ 36, Hbrecon, HHU 2, HAZ 29, HHP 88, HHU 3, HHU 4 and HGua), Steinernema intermedia, NC513 strain of S. glaserii, S. anomali, S. riobrave, Steinernema sp. and 5 strains of S. feltiae (22, Vija Norway, HU 1, scp, and IS 6). None of the examined EPN species or strains showed larvicidal efficacy at 37°C (no killing effect was observed in the case of the two heat-tolerant strains - H. bacteriophora and S. feltiae) against L. sericata larvae. At lower temperatures (20°C and 25°C) only strains of S. feltiae were found to be active. The overall odds ratios calculated for L. sericata maggots to contract S. feltiae nematode infection show significant (p <0.05) effect only in the case of strains HU 1, 22 and IS 6. In the case of strains HU 1 and 22 parasitic forms of S. feltiae could be detected in the dead larvae of L. sericata. Strain IS 6 (and also Vija Norway at 20°C) penetrated and killed fly larvae, but only adult forms of the nematode occurred in the cadavers.

Original languageEnglish
Pages (from-to)65-71
Number of pages7
JournalActa Veterinaria Hungarica
Volume53
Issue number1
DOIs
Publication statusPublished - 2005

Fingerprint

Lucilia sericata
nematode larvae
entomopathogenic nematodes
Calliphoridae
Diptera
Larva
Myiasis
Norway
myiasis
Steinernema
Heterorhabditis bacteriophora
Nematode Infections
Southern Africa
insect larvae
Livestock
larvae
New Zealand
Cadaver
Steinernema riobravis
Nematoda

Keywords

  • Blowfly
  • Entomopathogenic nematodes
  • Larvicidal efficacy
  • Lucilia sericata
  • Myiasis

ASJC Scopus subject areas

  • veterinary(all)

Cite this

@article{158a70fc841846ee83c4388f9123c488,
title = "Evaluation of efficacy of entomopathogenic nematodes against larvae of Lucilia sericata (Meigen, 1826) (Diptera: Calliphoridae)",
abstract = "The blowfly Lucilia sericata (Meigen, 1826) (Diptera: Calliphoridae) is the primary agent of cutaneous myiasis of sheep in northern Europe, southern Africa, Australia and New Zealand. As the application of chemicals has several disadvantages, alternative control measures of traumatic myiasis of livestock must be developed. In this study, the use of entomopathogenic nematodes (EPNs) as potential biocontrol agents against second instar larvae of Lucilia sericata was considered. The following nematode species were tested: Heterorhabditis bacteriophora (IS 5, HHU 1, Hmo1, HNC 1, HAZ 36, Hbrecon, HHU 2, HAZ 29, HHP 88, HHU 3, HHU 4 and HGua), Steinernema intermedia, NC513 strain of S. glaserii, S. anomali, S. riobrave, Steinernema sp. and 5 strains of S. feltiae (22, Vija Norway, HU 1, scp, and IS 6). None of the examined EPN species or strains showed larvicidal efficacy at 37°C (no killing effect was observed in the case of the two heat-tolerant strains - H. bacteriophora and S. feltiae) against L. sericata larvae. At lower temperatures (20°C and 25°C) only strains of S. feltiae were found to be active. The overall odds ratios calculated for L. sericata maggots to contract S. feltiae nematode infection show significant (p <0.05) effect only in the case of strains HU 1, 22 and IS 6. In the case of strains HU 1 and 22 parasitic forms of S. feltiae could be detected in the dead larvae of L. sericata. Strain IS 6 (and also Vija Norway at 20°C) penetrated and killed fly larvae, but only adult forms of the nematode occurred in the cadavers.",
keywords = "Blowfly, Entomopathogenic nematodes, Larvicidal efficacy, Lucilia sericata, Myiasis",
author = "E. T{\'o}th and K. M{\'a}rialigeti and A. Fodor and A. Lucskai and R. Farkas",
year = "2005",
doi = "10.1556/AVet.53.2005.1.7",
language = "English",
volume = "53",
pages = "65--71",
journal = "Acta Veterinaria Hungarica",
issn = "0236-6290",
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TY - JOUR

T1 - Evaluation of efficacy of entomopathogenic nematodes against larvae of Lucilia sericata (Meigen, 1826) (Diptera

T2 - Calliphoridae)

AU - Tóth, E.

AU - Márialigeti, K.

AU - Fodor, A.

AU - Lucskai, A.

AU - Farkas, R.

PY - 2005

Y1 - 2005

N2 - The blowfly Lucilia sericata (Meigen, 1826) (Diptera: Calliphoridae) is the primary agent of cutaneous myiasis of sheep in northern Europe, southern Africa, Australia and New Zealand. As the application of chemicals has several disadvantages, alternative control measures of traumatic myiasis of livestock must be developed. In this study, the use of entomopathogenic nematodes (EPNs) as potential biocontrol agents against second instar larvae of Lucilia sericata was considered. The following nematode species were tested: Heterorhabditis bacteriophora (IS 5, HHU 1, Hmo1, HNC 1, HAZ 36, Hbrecon, HHU 2, HAZ 29, HHP 88, HHU 3, HHU 4 and HGua), Steinernema intermedia, NC513 strain of S. glaserii, S. anomali, S. riobrave, Steinernema sp. and 5 strains of S. feltiae (22, Vija Norway, HU 1, scp, and IS 6). None of the examined EPN species or strains showed larvicidal efficacy at 37°C (no killing effect was observed in the case of the two heat-tolerant strains - H. bacteriophora and S. feltiae) against L. sericata larvae. At lower temperatures (20°C and 25°C) only strains of S. feltiae were found to be active. The overall odds ratios calculated for L. sericata maggots to contract S. feltiae nematode infection show significant (p <0.05) effect only in the case of strains HU 1, 22 and IS 6. In the case of strains HU 1 and 22 parasitic forms of S. feltiae could be detected in the dead larvae of L. sericata. Strain IS 6 (and also Vija Norway at 20°C) penetrated and killed fly larvae, but only adult forms of the nematode occurred in the cadavers.

AB - The blowfly Lucilia sericata (Meigen, 1826) (Diptera: Calliphoridae) is the primary agent of cutaneous myiasis of sheep in northern Europe, southern Africa, Australia and New Zealand. As the application of chemicals has several disadvantages, alternative control measures of traumatic myiasis of livestock must be developed. In this study, the use of entomopathogenic nematodes (EPNs) as potential biocontrol agents against second instar larvae of Lucilia sericata was considered. The following nematode species were tested: Heterorhabditis bacteriophora (IS 5, HHU 1, Hmo1, HNC 1, HAZ 36, Hbrecon, HHU 2, HAZ 29, HHP 88, HHU 3, HHU 4 and HGua), Steinernema intermedia, NC513 strain of S. glaserii, S. anomali, S. riobrave, Steinernema sp. and 5 strains of S. feltiae (22, Vija Norway, HU 1, scp, and IS 6). None of the examined EPN species or strains showed larvicidal efficacy at 37°C (no killing effect was observed in the case of the two heat-tolerant strains - H. bacteriophora and S. feltiae) against L. sericata larvae. At lower temperatures (20°C and 25°C) only strains of S. feltiae were found to be active. The overall odds ratios calculated for L. sericata maggots to contract S. feltiae nematode infection show significant (p <0.05) effect only in the case of strains HU 1, 22 and IS 6. In the case of strains HU 1 and 22 parasitic forms of S. feltiae could be detected in the dead larvae of L. sericata. Strain IS 6 (and also Vija Norway at 20°C) penetrated and killed fly larvae, but only adult forms of the nematode occurred in the cadavers.

KW - Blowfly

KW - Entomopathogenic nematodes

KW - Larvicidal efficacy

KW - Lucilia sericata

KW - Myiasis

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