Evaluation of column characteristics in RPLC using linear solvation energy relationships (LSERs)

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

An overview is given of our studies on the use of linear solvation energy relationships (LSERs) for evaluation and comparison of RP-HPLC columns. From among the columns investigated, 19 columns of different types, viz. 12 narrow-pore, 7 wide-pore silicas containing various ligands (C18, C8, C4, CN) and obtained from 7 manufacturers, were compared and evaluated. Retention factors of 32 solutes of widely differing chemical properties were measured and evaluated to determine the effect of stationary phase properties on the retention of various types of solutes. When the same mobile phase is used with the different columns, the regression coefficients determined by multivariate regression characterize the various stationary phases. The columns investigated were evaluated on the basis of the relative importance of the various types of interactions reflecting the various surface properties of the stationary phases. Since the selectivity of separation is more important than is absolute selectivity, we have investigated and compared the various selectivity terms, hydrophobic, polar, and specific selectivity, displayed by the various columns, for the separation of different types of compounds. As the selectivity of separation depends also on the type and composition of the mobile phase, the effect of mobile phase on the various selectivity terms was evaluated.

Original languageEnglish
Pages (from-to)201-214
Number of pages14
JournalJournal of Separation Science
Volume26
Issue number3-4
DOIs
Publication statusPublished - Mar 2003

Fingerprint

Surface Properties
Solvation
Silicon Dioxide
High Pressure Liquid Chromatography
Ligands
Chemical properties
Surface properties
Silica
Chemical analysis

Keywords

  • Linear solvation energy relationships
  • Narrow pore and wide pore packings
  • Retention and selectivity factors
  • Various ligands

ASJC Scopus subject areas

  • Molecular Medicine
  • Clinical Biochemistry
  • Chemical Engineering(all)
  • Filtration and Separation

Cite this

Evaluation of column characteristics in RPLC using linear solvation energy relationships (LSERs). / Szepesy, L.

In: Journal of Separation Science, Vol. 26, No. 3-4, 03.2003, p. 201-214.

Research output: Contribution to journalArticle

@article{e229038a18d04534ba4c4fb37170d6cf,
title = "Evaluation of column characteristics in RPLC using linear solvation energy relationships (LSERs)",
abstract = "An overview is given of our studies on the use of linear solvation energy relationships (LSERs) for evaluation and comparison of RP-HPLC columns. From among the columns investigated, 19 columns of different types, viz. 12 narrow-pore, 7 wide-pore silicas containing various ligands (C18, C8, C4, CN) and obtained from 7 manufacturers, were compared and evaluated. Retention factors of 32 solutes of widely differing chemical properties were measured and evaluated to determine the effect of stationary phase properties on the retention of various types of solutes. When the same mobile phase is used with the different columns, the regression coefficients determined by multivariate regression characterize the various stationary phases. The columns investigated were evaluated on the basis of the relative importance of the various types of interactions reflecting the various surface properties of the stationary phases. Since the selectivity of separation is more important than is absolute selectivity, we have investigated and compared the various selectivity terms, hydrophobic, polar, and specific selectivity, displayed by the various columns, for the separation of different types of compounds. As the selectivity of separation depends also on the type and composition of the mobile phase, the effect of mobile phase on the various selectivity terms was evaluated.",
keywords = "Linear solvation energy relationships, Narrow pore and wide pore packings, Retention and selectivity factors, Various ligands",
author = "L. Szepesy",
year = "2003",
month = "3",
doi = "10.1002/jssc.200390027",
language = "English",
volume = "26",
pages = "201--214",
journal = "Journal of Separation Science",
issn = "1615-9306",
publisher = "Wiley-VCH Verlag",
number = "3-4",

}

TY - JOUR

T1 - Evaluation of column characteristics in RPLC using linear solvation energy relationships (LSERs)

AU - Szepesy, L.

PY - 2003/3

Y1 - 2003/3

N2 - An overview is given of our studies on the use of linear solvation energy relationships (LSERs) for evaluation and comparison of RP-HPLC columns. From among the columns investigated, 19 columns of different types, viz. 12 narrow-pore, 7 wide-pore silicas containing various ligands (C18, C8, C4, CN) and obtained from 7 manufacturers, were compared and evaluated. Retention factors of 32 solutes of widely differing chemical properties were measured and evaluated to determine the effect of stationary phase properties on the retention of various types of solutes. When the same mobile phase is used with the different columns, the regression coefficients determined by multivariate regression characterize the various stationary phases. The columns investigated were evaluated on the basis of the relative importance of the various types of interactions reflecting the various surface properties of the stationary phases. Since the selectivity of separation is more important than is absolute selectivity, we have investigated and compared the various selectivity terms, hydrophobic, polar, and specific selectivity, displayed by the various columns, for the separation of different types of compounds. As the selectivity of separation depends also on the type and composition of the mobile phase, the effect of mobile phase on the various selectivity terms was evaluated.

AB - An overview is given of our studies on the use of linear solvation energy relationships (LSERs) for evaluation and comparison of RP-HPLC columns. From among the columns investigated, 19 columns of different types, viz. 12 narrow-pore, 7 wide-pore silicas containing various ligands (C18, C8, C4, CN) and obtained from 7 manufacturers, were compared and evaluated. Retention factors of 32 solutes of widely differing chemical properties were measured and evaluated to determine the effect of stationary phase properties on the retention of various types of solutes. When the same mobile phase is used with the different columns, the regression coefficients determined by multivariate regression characterize the various stationary phases. The columns investigated were evaluated on the basis of the relative importance of the various types of interactions reflecting the various surface properties of the stationary phases. Since the selectivity of separation is more important than is absolute selectivity, we have investigated and compared the various selectivity terms, hydrophobic, polar, and specific selectivity, displayed by the various columns, for the separation of different types of compounds. As the selectivity of separation depends also on the type and composition of the mobile phase, the effect of mobile phase on the various selectivity terms was evaluated.

KW - Linear solvation energy relationships

KW - Narrow pore and wide pore packings

KW - Retention and selectivity factors

KW - Various ligands

UR - http://www.scopus.com/inward/record.url?scp=0037354350&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037354350&partnerID=8YFLogxK

U2 - 10.1002/jssc.200390027

DO - 10.1002/jssc.200390027

M3 - Article

VL - 26

SP - 201

EP - 214

JO - Journal of Separation Science

JF - Journal of Separation Science

SN - 1615-9306

IS - 3-4

ER -