Evaluation of chlorophyll fluorescence and membrane injury in the leaves of barley cultivars under osmotic stress

Konstantina Kocheva, P. Lambrev, Georgy Georgiev, Vasilii Goltsev, Miroslav Karabaliev

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

Two physiological tests for screening drought tolerance of barley (Hordeum vulgare, L.) plants are compared in this work. Water deficit is induced by treating the plants' roots with polyethylene glycol (PEG 8000). The relative water content (RWC) of the plants is used as a measure of the water status. Conductometrically determined electrolyte leakage from the leaf tissue demonstrates the membrane injury caused by dehydration. It is shown that the injury index increases with the decrease of the RWC of the leaves. The F v/Fm ratio is employed to assess changes in the primary photochemical reactions of the photosynthetic apparatus after dehydration. The results suggest that PSII is weakly affected by the imposed osmotic stress. The fluorescence behaviour of the examined cultivars is related to their RWC.

Original languageEnglish
Pages (from-to)121-124
Number of pages4
JournalBioelectrochemistry
Volume63
Issue number1-2
DOIs
Publication statusPublished - Jun 2004

Fingerprint

barley
Osmotic Pressure
chlorophylls
Chlorophyll
Hordeum
leaves
Water content
moisture content
Fluorescence
membranes
Membranes
Dehydration
dehydration
fluorescence
physiological tests
Polyethylene glycols
Water
evaluation
Wounds and Injuries
plant roots

Keywords

  • Barley
  • Chlorophyll fluorescence
  • Electrolyte leakage
  • PEG 8000
  • PSII

ASJC Scopus subject areas

  • Biophysics
  • Electrochemistry
  • Physical and Theoretical Chemistry

Cite this

Evaluation of chlorophyll fluorescence and membrane injury in the leaves of barley cultivars under osmotic stress. / Kocheva, Konstantina; Lambrev, P.; Georgiev, Georgy; Goltsev, Vasilii; Karabaliev, Miroslav.

In: Bioelectrochemistry, Vol. 63, No. 1-2, 06.2004, p. 121-124.

Research output: Contribution to journalArticle

Kocheva, Konstantina ; Lambrev, P. ; Georgiev, Georgy ; Goltsev, Vasilii ; Karabaliev, Miroslav. / Evaluation of chlorophyll fluorescence and membrane injury in the leaves of barley cultivars under osmotic stress. In: Bioelectrochemistry. 2004 ; Vol. 63, No. 1-2. pp. 121-124.
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