Evaluation of boron neutron capture effects in cell culture using sulforhodamine-B assay and a colony assay

A. Wittig, W. Sauerwein, F. Pöller, C. Fuhrmann, K. Hideghéty, C. Streffer

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Purpose: The purpose of this study was to find an in vitro method for determining the cytotoxicity of boronated drugs as well as their potential suitability for neutron capture therapy. Materials and methods: The survival of human melanoma cells has been determined by a colony assay and the sulforhodamine-B assay after X-irradiation and irradiation with fast d(14) + Beneutrons using the boronated compound borocaptate sodium (BSH). The cytotoxic effects of BSH have been studied using both methods. Results: Under well-defined experimental conditions, and after a sufficient amount of time for the expression of radiation damage, the results of the sulforhodamine-B assay are qualitatively comparable with the results of the colony assay. Conclusion: The sulforhodamine-B assay is suitable for the screening of compounds for potential use in neutron capture therapy because it is a fast and efficient method that is reproducible and technically advantageous.

Original languageEnglish
Pages (from-to)679-690
Number of pages12
JournalInternational Journal of Radiation Biology
Volume73
Issue number6
DOIs
Publication statusPublished - 1998

Fingerprint

lissamine rhodamine B
capture effect
Boron
Neutrons
boron
Cell culture
therapy
Assays
cell culture
Neutron Capture Therapy
Cell Culture Techniques
sodium compounds
neutrons
irradiation
evaluation
assays
radiation damage
drugs
screening
cytotoxicity

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology
  • Nuclear Energy and Engineering
  • Radiation

Cite this

Evaluation of boron neutron capture effects in cell culture using sulforhodamine-B assay and a colony assay. / Wittig, A.; Sauerwein, W.; Pöller, F.; Fuhrmann, C.; Hideghéty, K.; Streffer, C.

In: International Journal of Radiation Biology, Vol. 73, No. 6, 1998, p. 679-690.

Research output: Contribution to journalArticle

Wittig, A. ; Sauerwein, W. ; Pöller, F. ; Fuhrmann, C. ; Hideghéty, K. ; Streffer, C. / Evaluation of boron neutron capture effects in cell culture using sulforhodamine-B assay and a colony assay. In: International Journal of Radiation Biology. 1998 ; Vol. 73, No. 6. pp. 679-690.
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