Evaluation of blood lactate and plasma insulin during high-intensity exercise by antecubital vein catheterization

Minas Nalbandian, Zsolt Radak, Masaki Takeda

Research output: Contribution to journalArticle


The measurement of metabolic and endocrinal markers during physical activity is of relevance to understanding the physiological implications of different exercise modalities. During some exercise modalities (e.g., high-intensity interval exercise), blood metabolites and hormonal levels change in short periods of time. In the present study, we describe a method to catheterize the antecubital vein, which allows the collection of several blood samples during exercise. Insulin and venous lactate concentrations were measured during high-intensity exercise by the application of the described method. The exercise consisted of three 30 s bouts of high-intensity exercise separated by 4 min of recovery. After the last recovery period, a Wingate test was performed. Blood samples from the antecubital vein were obtained before and after each 30 s bout and before and after the Wingate test. As a result, it was possible to evaluate the plasma insulin and venous blood lactate variations during the exercise.

Original languageEnglish
Article numbere56890
JournalJournal of Visualized Experiments
Issue number135
Publication statusPublished - May 18 2018


  • Antecubital vein catheterization
  • Blood sampling
  • Exercise testing
  • High-intensity interval exercise
  • Hormones
  • Immunology and Infection
  • Issue 135
  • Wingate test

ASJC Scopus subject areas

  • Neuroscience(all)
  • Chemical Engineering(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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