Evaluation of an enzyme immunoassay for the detection of the insect growth regulator fenoxycarb in environmental and biological samplest

Hong T M Le, Ferenc Szurdoki, András Székács

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

A competitive enzyme-linked immunosorbent assay (ELISA) for fenoxycarb was adapted for quantitative detection of this insect growth regulator in various environmental, agricultural, food and biological matrices. Environmental samples were taken from soil and surface waters in Hungary. The ELISA enabled fenoxycarb detection in surface waters in the 1.1-125ng ml-1 concentration range without sample cleanup. In contrast, soil produced a strong matrix effect due to humic acids and other soil components. Several fruit homogenates and commercial fruit juices (eg apple, pear, grape) were analyzed by the ELISA. The assay was found to be suitable for analysis of fenoxycarb in fruit juices diluted 1:40. Biological samples included insect, fish and bovine tissues. The ELISA was applied to detect fenoxycarb in various biological matrices from larvae of the silkworm, Bombyx mori L. The assay proved useful for the analysis of haemolymph diluted 1:10 or at higher dilutions. Fat body and whole body homogenates, however, caused severe matrix effects. Fenoxycarb was detected in liver homogenates (diluted 1:40) from fish treated with various doses of fenoxycarb, and the concentrations determined correlated with the applied doses. The method was used to analyze spiked bovine urine samples diluted 1:10 or at greater dilutions. Fenoxycarb content determined by the ELISA in water and fruit juice samples was validated using GC-MS with solid-phase microextraction (SPME) sample preparation. The results of these studies demonstrated both the value and limitations of the assay when used for monitoring fenoxycarb in environmental, food and biological samples.

Original languageEnglish
Pages (from-to)410-416
Number of pages7
JournalPest Management Science
Volume59
Issue number4
DOIs
Publication statusPublished - Apr 1 2003

Fingerprint

fenoxycarb
insect growth regulators
enzyme immunoassays
enzyme-linked immunosorbent assay
fruit juices
sampling
surface water
assays
cattle
humic acids
Bombyx mori
dosage
fish
Hungary
pears
hemolymph
insect larvae
grapes
soil
urine

Keywords

  • Biological tissues
  • Bombyx mori
  • Bovine urine
  • ELISA
  • Fenoxycarb
  • Fish liver
  • Fruit juice
  • Haemolymph
  • Water, soil, food

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Insect Science

Cite this

Evaluation of an enzyme immunoassay for the detection of the insect growth regulator fenoxycarb in environmental and biological samplest. / Le, Hong T M; Szurdoki, Ferenc; Székács, András.

In: Pest Management Science, Vol. 59, No. 4, 01.04.2003, p. 410-416.

Research output: Contribution to journalArticle

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