Evaluation of a wide range of pepper genotypes for regeneration and transformation with an Agrobacterium tumefaciens shooter strain

E. Balázs, Á Bukovinszki, M. Csányi, G. Csilléry, Z. Divéki, I. Nagy, J. Mitykó, K. Salánki, V. Mihálka

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

A regeneration protocol developed for the Agrobacterium tumefaciens-mediated transformation of pepper (Capsicum annuum L.) was used to evaluate the potential for genetic transformation of 107 doubled haploid (DH) pepper genotypes belonging to 12 main cultivar groups. The genotypes were scored on the basis of the ratio of regenerated shoots compared to the commercial cultivar Fehérözön, which exhibited 30-70% regeneration from the total number of explants. Fifty DH genotypes responded, representing all the of the main cultivar groups, 31 of which showed regeneration frequencies similar to or better than cv. Fehérözön. By using a shooter strain with a binary vector harbouring the cucumber mosaic virus (CMV) coat protein (CP) gene and selecting regenerants on media containing kanamycin, the transformability of the pepper genotypes as well as resistance to CMV were simultaneously analyzed. The regenerated plants were selected and tested by PCR to detect the CP gene. Eighteen PCR-positive DH plants and six PCR-positive commercial Fehérözön cultivar plants were regenerated and rooted by grafting. In the case of three transgenic genotypes, presence of the CMV CP was confirmed by PCR in the T1 generation indicating the stable integration of the CP gene. In the progeny of one DH genotype of the Demre type, the presence of the transgene as well as resistance to the virus were demonstrated in the T2 generation.

Original languageEnglish
Pages (from-to)720-725
Number of pages6
JournalSouth African Journal of Botany
Volume74
Issue number4
DOIs
Publication statusPublished - Nov 2008

Fingerprint

doubled haploids
coat proteins
Agrobacterium radiobacter
pepper
Cucumber mosaic virus
genotype
cultivars
haplotypes
genes
genetic transformation
grafting (plants)
kanamycin
Capsicum annuum
transgenes
explants
genetically modified organisms
viruses
shoots

Keywords

  • Doubled haploid transgenic cultivars
  • Pepper regeneration
  • Transgenic virus resistance

ASJC Scopus subject areas

  • Plant Science

Cite this

Evaluation of a wide range of pepper genotypes for regeneration and transformation with an Agrobacterium tumefaciens shooter strain. / Balázs, E.; Bukovinszki, Á; Csányi, M.; Csilléry, G.; Divéki, Z.; Nagy, I.; Mitykó, J.; Salánki, K.; Mihálka, V.

In: South African Journal of Botany, Vol. 74, No. 4, 11.2008, p. 720-725.

Research output: Contribution to journalArticle

Balázs, E. ; Bukovinszki, Á ; Csányi, M. ; Csilléry, G. ; Divéki, Z. ; Nagy, I. ; Mitykó, J. ; Salánki, K. ; Mihálka, V. / Evaluation of a wide range of pepper genotypes for regeneration and transformation with an Agrobacterium tumefaciens shooter strain. In: South African Journal of Botany. 2008 ; Vol. 74, No. 4. pp. 720-725.
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