Evaluation of a novel magneto-optical method for the detection of malaria parasites

Ágnes Orbán, Ádám Butykai, András Molnár, Zsófia Pröhle, Gergö Fülöp, T. Zelles, Wasan Forsyth, Danika Hill, Ivo Müller, Louis Schofield, Maria Rebelo, Thomas Hänscheid, Stephan Karl, I. Kézsmárki

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Improving the efficiency of malaria diagnosis is one of the main goals of current malaria research. We have recently developed a magneto-optical (MO) method which allows high-sensitivity detection of malaria pigment (hemozoin crystals) in blood via the magnetically induced rotational motion of the hemozoin crystals. Here, we evaluate this MO technique for the detection of Plasmodium falciparum in infected erythrocytes using in-vitro parasite cultures covering the entire intraerythrocytic life cycle. Our novel method detected parasite densities as low as ∼40 parasites per microliter of blood (0.0008% parasitemia) at the ring stage and less than 10 parasites/μL (0.0002% parasitemia) in the case of the later stages. These limits of detection, corresponding to approximately 20 pg/μL of hemozoin produced by the parasites, exceed that of rapid diagnostic tests and compete with the threshold achievable by light microscopic observation of blood smears. The MO diagnosis requires no special training of the operator or specific reagents for parasite detection, except for an inexpensive lysis solution to release intracellular hemozoin. The devices can be designed to a portable format for clinical and in-field tests. Besides testing its diagnostic performance, we also applied the MO technique to investigate the change in hemozoin concentration during parasite maturation. Our preliminary data indicate that this method may offer an efficient tool to determine the amount of hemozoin produced by the different parasite stages in synchronized cultures. Hence, it could eventually be used for testing the susceptibility of parasites to antimalarial drugs.

Original languageEnglish
Article numbere96981
JournalPLoS One
Volume9
Issue number5
DOIs
Publication statusPublished - May 13 2014

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malaria
Malaria
Parasites
parasites
Blood
Parasitemia
methodology
parasitemia
crystals
blood
Crystals
antimalarials
testing
Antimalarials
Plasmodium falciparum
Testing
hemozoin
Life Cycle Stages
Routine Diagnostic Tests
diagnostic techniques

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Evaluation of a novel magneto-optical method for the detection of malaria parasites. / Orbán, Ágnes; Butykai, Ádám; Molnár, András; Pröhle, Zsófia; Fülöp, Gergö; Zelles, T.; Forsyth, Wasan; Hill, Danika; Müller, Ivo; Schofield, Louis; Rebelo, Maria; Hänscheid, Thomas; Karl, Stephan; Kézsmárki, I.

In: PLoS One, Vol. 9, No. 5, e96981, 13.05.2014.

Research output: Contribution to journalArticle

Orbán, Á, Butykai, Á, Molnár, A, Pröhle, Z, Fülöp, G, Zelles, T, Forsyth, W, Hill, D, Müller, I, Schofield, L, Rebelo, M, Hänscheid, T, Karl, S & Kézsmárki, I 2014, 'Evaluation of a novel magneto-optical method for the detection of malaria parasites', PLoS One, vol. 9, no. 5, e96981. https://doi.org/10.1371/journal.pone.0096981
Orbán, Ágnes ; Butykai, Ádám ; Molnár, András ; Pröhle, Zsófia ; Fülöp, Gergö ; Zelles, T. ; Forsyth, Wasan ; Hill, Danika ; Müller, Ivo ; Schofield, Louis ; Rebelo, Maria ; Hänscheid, Thomas ; Karl, Stephan ; Kézsmárki, I. / Evaluation of a novel magneto-optical method for the detection of malaria parasites. In: PLoS One. 2014 ; Vol. 9, No. 5.
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