Ether lipid composition and molecular species alterations in carp brain (Cyprinus carpio L.) During normoxic temperature acclimation

Young K. Yeo, Eek J. Park, Chul W. Lee, Han T. Joo, Tibor Farkas

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Carp (Cyprinus carpio L.) whole brain was used to investigate the thermal acclimation changes under normoxic conditions of three-subclasses (alkenylacyl-, alkylacyl- and diacyl-subclasses) of choline glycerophospholipids (CGP), ethanolamine glycerophospholipids (EGP) and inositol glycerophospholipids (IGP) as well as their acyl chain profiles and molecular species composition. The alkanylacyl subclass of CGP and IGP and the alkylacyl subclass of CGP and EGP varied significantly during summer (25°C) acclimation compared to winter (5°C). The levels of alkenylacyl and alkylacyl-CGP, alkylacyl-EGP and alkenylacyl-IGP were 17.3-, 3.7-, 3.5- and 1.3-fold higher in the summer, respectively, while the alkenylacyl EGP was moderately lower. The levels of diacyl subclasses from CGP and IGP were considerably lower in the summer to compensate for the higher proportion of alkenylacyl and alkylacyl subclasses. Significant changes of ether phospholipids and the reorganization of the molecular species composition of all lipid subclasses may be associated with the 'fine tuning' of the physical properties of the cellular membranes in carp brain due to temperature acclimation. The overall acyl chain profile of the three subclasses of carp brain phospholipids showed differences in composition depending upon the subclass of the individual phospholipid. Generally, the polyunsaturated fatty acid (PUFA) chain composition increased relative to monounsaturated fatty acid (MUFA) and saturated fatty acids (SFA) during winter acclimation. Docosahexaenoic acid (DHA) was richer in the winter compared to summer. However, no DHA was found in ether-containing species of IGP from either winter or summer, except for 2% in alkylacyl-IGP during the summer. The above observations suggest that the content of ether phospholipids (alkenylacyl and alkylacyl) as well as the reorganization of the molecular species composition of all phospholipids may serve to maintain a functional fluid-crystalline state to preserve the signaling functions in carp brain.

Original languageEnglish
Pages (from-to)1257-1264
Number of pages8
JournalNeurochemical research
Volume22
Issue number10
DOIs
Publication statusPublished - Oct 25 1997

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Keywords

  • Brain
  • Ether lipids
  • Fatty acids
  • Fish
  • Phospholipid molecular species
  • Plasmalogens
  • Temperature acclimation

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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