Primary cultured bovine adrenal medullary chromaffin cells were used to study the effects of ethanol on inositol phosphate accumulation and protein kinase C (PKC) activity in neuronal cells. Ethanol (50 and 200 mM) exposure for 30 min activates PKC in bovine adrenal chromaffin cells. Chronic exposure of bovine adrenal medullary chromaffin cells to ethanol (100 and 200 mM) maintains the activation of PKC over a 24 h period. The ethanol-induced (200 mM) activation of PKC at 24 h was visualized by Western blot using antisera specific to the PKCα and -ε isoenzymes. Only a marginal increase in inositol phosphate accumulation was observed during a 60 min exposure to ethanol (200 mM) as compared to histamine (100 μM). Shorter exposures had no effect. Therefore, the activation of PKC may not be solely due to increased production of diacylglycerol from phosphoinositides. Rather it may be due to ethanol-induced changes in membrane properties of chromaffin cells.
|Number of pages||3|
|Journal||Pharmaceutical and Pharmacological Letters|
|Publication status||Published - Jan 1 1998|
ASJC Scopus subject areas
- Pharmaceutical Science