Ethanol and halothane differently modulate HLA class I and class II oligomerization. A new look at the mode of action of anesthetic agents through fluorescence spectroscopy

M. Dzoljic, L. Bene, Z. Krasznai, S. Damjanovich, B. Van Duijn

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The field of research considering the working mechanism of anesthetic agents is a complex one and the site or sites of action of general anesthetics are yet to be elucidated. Through the years, on the molecular level, the discussion has shifted from the lipid theories to the more specific interaction with the proteins responsible for the signal transduction. While this approach led to several models, they offer, at best, partial explanations for the observed phenomena. Anesthetic agents interact with many systems, of which the neuronal is best studied, leaving interaction with the immune defense system relatively unexplored. In this study we focus on the interaction of ethanol and halothane with the co-localization on the membrane of HLA I and II molecules. We show that ethanol tends to randomize the distribution of HLA I and II molecules, while halothane increases the clustering of HLA I proteins. The notion that anesthetics modulate cell function by disrupting clustering and thereby promoting a random distribution is a novel approach that may explain the general involvement of many systems during exposition to anesthetic drugs. In this study we show the disturbance of co-localization of molecules that may form a functional network. The relevance of this finding depends on the importance of these networks for extracellular and intracellular processes. (C) 2000 Elsevier Science S.A. All rights reserved.

Original languageEnglish
Pages (from-to)48-52
Number of pages5
JournalJournal of Photochemistry and Photobiology, B: Biology
Volume56
Issue number1
DOIs
Publication statusPublished - 2000

Fingerprint

fluorescence emission spectroscopy
anesthetics
Anesthetics
Oligomerization
Fluorescence Spectrometry
Fluorescence spectroscopy
halothane
Halothane
mechanism of action
Ethanol
ethyl alcohol
ethanol
fluorescence
spectroscopy
Molecules
Cluster Analysis
general anesthetics
Signal transduction
General Anesthetics
proteins

Keywords

  • Anesthetic agents
  • Cell surface proteins
  • Ethanol
  • Fluorescence spectroscopy
  • Halothane

ASJC Scopus subject areas

  • Plant Science
  • Bioengineering
  • Physical and Theoretical Chemistry

Cite this

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abstract = "The field of research considering the working mechanism of anesthetic agents is a complex one and the site or sites of action of general anesthetics are yet to be elucidated. Through the years, on the molecular level, the discussion has shifted from the lipid theories to the more specific interaction with the proteins responsible for the signal transduction. While this approach led to several models, they offer, at best, partial explanations for the observed phenomena. Anesthetic agents interact with many systems, of which the neuronal is best studied, leaving interaction with the immune defense system relatively unexplored. In this study we focus on the interaction of ethanol and halothane with the co-localization on the membrane of HLA I and II molecules. We show that ethanol tends to randomize the distribution of HLA I and II molecules, while halothane increases the clustering of HLA I proteins. The notion that anesthetics modulate cell function by disrupting clustering and thereby promoting a random distribution is a novel approach that may explain the general involvement of many systems during exposition to anesthetic drugs. In this study we show the disturbance of co-localization of molecules that may form a functional network. The relevance of this finding depends on the importance of these networks for extracellular and intracellular processes. (C) 2000 Elsevier Science S.A. All rights reserved.",
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AU - Damjanovich, S.

AU - Van Duijn, B.

PY - 2000

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