Etest for assessing the susceptibility of filamentous fungi

Ilona Dóczi, Erika Dósa, J. Varga, Zsuzsanna Antal, L. Kredics, Elizabeth Nagy

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The purpose of this study was to evaluate the Etest as an in vitro antifungal susceptibility test method for different moulds originating from human samples and from the environment. A total of 50 isolates (1 Acremonium, 18 Aspergillus, 2 Cladosporium, 1 Epicoccum, 15 Penicillium, 2 Scopulariopsis and 11 Trichoderma strains) were tested by the Etest. Forty-six of the tested moulds (92%) were resistant to fluconazole with minimal inhibitory concentrations (MICs) ≥ 256 μg ml-1. There were strains resistant to ketoconazole among Aspergillus niger, A. ochraceus and Cladosporium spp. with MICs > 32 μg ml-1. For fluconazole, no differences were observed using two different inocula, while for itraconazole, ketoconazole and amphotericin B, a 1 or less step 2-fold dilution difference in MIC was seen for the most of 10 selected strains. The MICs of fluconazole and amphotericin B obtained for Trichoderma strains by the Etest and the agar dilution method were also compared. MICs for fluconazole were in agreement, while MICs for amphotericin B were higher with 1 or 2 steps of 2-fold dilutions for most of Trichoderma strains in the case of the agar dilution method.

Original languageEnglish
Pages (from-to)271-281
Number of pages11
JournalActa microbiologica et immunologica Hungarica
Volume51
Issue number3
DOIs
Publication statusPublished - Dec 1 2004

Keywords

  • Agar dilution
  • Antifungal susceptibility testing
  • Etest
  • Minimal inhibitory concentration
  • Moulds

ASJC Scopus subject areas

  • Immunology and Microbiology(all)

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