Estrogen replacement therapy reverses changes in intramural coronary resistance arteries caused by female sex hormone depletion

Metin Mericli, G. Nádasy, Maria Szekeres, S. Várbíró, Z. Vajó, Máté Mátrai, N. Ács, E. Monos, B. Székács

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18 Citations (Scopus)

Abstract

Objective: We tested the hypothesis that female sex hormone depletion and estradiol replacement therapy significantly influences the biomechanical properties of intramural coronary resistance arteries. Design: Female rats (n=30) were divided into three groups. In group O, rats were subjected to bilateral ovariectomy. Group HRT was subjected to bilateral ovariectomy and estradiol replacement therapy. Rats in group C served as controls. One month after ovariectomy, intramural coronary arteries (approximately 200 μm in diameter) branching from the left anterior descending coronary were isolated, cannulated and studied by microarteriography. Intraluminal pressure was increased in steps between 0 and 90 mm Hg. The steady state diameter at each step was measured. These measurements were repeated in the presence of U46619, a thromboxane (TX) A2 receptor agonist (at a concentration of 10 -6 M), and bradykinin (BK; at 10-6 M). Finally, Ca 2+-free Krebs-induced passive diameter (PD) was measured in each group. Results: Ovariectomy increased spontaneous myogenic tone of coronary arteries (p2 agonist induced the largest contraction in the ovariectomized group, whereas bradykinin-induced relaxation was the largest in the estrogen replacement group (p

Original languageEnglish
Pages (from-to)317-324
Number of pages8
JournalCardiovascular Research
Volume61
Issue number2
DOIs
Publication statusPublished - Feb 1 2004

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Estrogen Replacement Therapy
Gonadal Steroid Hormones
Ovariectomy
Coronary Vessels
Bradykinin
Estradiol
Prostaglandin H2 Receptors Thromboxane A2
15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid
Pressure
Therapeutics

Keywords

  • Contractility
  • Coronary artery
  • Endothelium
  • Estrogen
  • Vessel wall

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

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title = "Estrogen replacement therapy reverses changes in intramural coronary resistance arteries caused by female sex hormone depletion",
abstract = "Objective: We tested the hypothesis that female sex hormone depletion and estradiol replacement therapy significantly influences the biomechanical properties of intramural coronary resistance arteries. Design: Female rats (n=30) were divided into three groups. In group O, rats were subjected to bilateral ovariectomy. Group HRT was subjected to bilateral ovariectomy and estradiol replacement therapy. Rats in group C served as controls. One month after ovariectomy, intramural coronary arteries (approximately 200 μm in diameter) branching from the left anterior descending coronary were isolated, cannulated and studied by microarteriography. Intraluminal pressure was increased in steps between 0 and 90 mm Hg. The steady state diameter at each step was measured. These measurements were repeated in the presence of U46619, a thromboxane (TX) A2 receptor agonist (at a concentration of 10 -6 M), and bradykinin (BK; at 10-6 M). Finally, Ca 2+-free Krebs-induced passive diameter (PD) was measured in each group. Results: Ovariectomy increased spontaneous myogenic tone of coronary arteries (p2 agonist induced the largest contraction in the ovariectomized group, whereas bradykinin-induced relaxation was the largest in the estrogen replacement group (p",
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T1 - Estrogen replacement therapy reverses changes in intramural coronary resistance arteries caused by female sex hormone depletion

AU - Mericli, Metin

AU - Nádasy, G.

AU - Szekeres, Maria

AU - Várbíró, S.

AU - Vajó, Z.

AU - Mátrai, Máté

AU - Ács, N.

AU - Monos, E.

AU - Székács, B.

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N2 - Objective: We tested the hypothesis that female sex hormone depletion and estradiol replacement therapy significantly influences the biomechanical properties of intramural coronary resistance arteries. Design: Female rats (n=30) were divided into three groups. In group O, rats were subjected to bilateral ovariectomy. Group HRT was subjected to bilateral ovariectomy and estradiol replacement therapy. Rats in group C served as controls. One month after ovariectomy, intramural coronary arteries (approximately 200 μm in diameter) branching from the left anterior descending coronary were isolated, cannulated and studied by microarteriography. Intraluminal pressure was increased in steps between 0 and 90 mm Hg. The steady state diameter at each step was measured. These measurements were repeated in the presence of U46619, a thromboxane (TX) A2 receptor agonist (at a concentration of 10 -6 M), and bradykinin (BK; at 10-6 M). Finally, Ca 2+-free Krebs-induced passive diameter (PD) was measured in each group. Results: Ovariectomy increased spontaneous myogenic tone of coronary arteries (p2 agonist induced the largest contraction in the ovariectomized group, whereas bradykinin-induced relaxation was the largest in the estrogen replacement group (p

AB - Objective: We tested the hypothesis that female sex hormone depletion and estradiol replacement therapy significantly influences the biomechanical properties of intramural coronary resistance arteries. Design: Female rats (n=30) were divided into three groups. In group O, rats were subjected to bilateral ovariectomy. Group HRT was subjected to bilateral ovariectomy and estradiol replacement therapy. Rats in group C served as controls. One month after ovariectomy, intramural coronary arteries (approximately 200 μm in diameter) branching from the left anterior descending coronary were isolated, cannulated and studied by microarteriography. Intraluminal pressure was increased in steps between 0 and 90 mm Hg. The steady state diameter at each step was measured. These measurements were repeated in the presence of U46619, a thromboxane (TX) A2 receptor agonist (at a concentration of 10 -6 M), and bradykinin (BK; at 10-6 M). Finally, Ca 2+-free Krebs-induced passive diameter (PD) was measured in each group. Results: Ovariectomy increased spontaneous myogenic tone of coronary arteries (p2 agonist induced the largest contraction in the ovariectomized group, whereas bradykinin-induced relaxation was the largest in the estrogen replacement group (p

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KW - Endothelium

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