Estrogen-mediated up-regulation of the Ca-dependent constitutive nitric oxide synthase in the rat aorta and heart

E. Morschl, Ildikó Bretus, János Nemcsik, F. László, Imre Pávó

Research output: Contribution to journalArticle

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Abstract

The role of endogenous estrogens has been studied in the regulation of the Ca-dependent constitutive nitric oxide synthase (cNOS) enzyme activity in aortic and cardiac tissues of the rat. The activity of cNOS enzyme was measured by the citrulline assay in the abdominal aorta and in the left ventricle of the heart obtained from male, sham-operated female and ovariectomized female Wistar rats. Estrogen replacement therapy (17-β-estradiol, 20-100 μg/kg/day, s.c.) has been performed in ovariectomized rats over two weeks. We found that cNOS activity was higher in the aorta and heart of female rats compared to males. Ovariectomy decreased cNOS activity in both tissues to that level what could be observed in males. Estrogen supplementation caused a dose-dependent elevation of cNOS enzyme activity in cardiac and aortic tissues, where the higher dose (100 μg) completely restored cNOS enzyme activity to the levels found in females. We concluded that endogenous estrogens up-regulate the activity of the cNOS isoenzymes in the rat aorta and heart. (C) 2000 Elsevier Science Inc.

Original languageEnglish
Pages (from-to)49-55
Number of pages7
JournalLife Sciences
Volume68
Issue number1
DOIs
Publication statusPublished - Nov 24 2000

Fingerprint

Nitric Oxide Synthase
Aorta
Rats
Estrogens
Up-Regulation
Enzyme activity
Enzymes
Tissue
Citrulline
Estrogen Replacement Therapy
Abdominal Aorta
Ovariectomy
Isoenzymes
Heart Ventricles
Wistar Rats
Estradiol
Assays

Keywords

  • Cardiovascular protection
  • ENOS enzyme regulation
  • Estrogen
  • Ischemic heart disease
  • Nitric oxide

ASJC Scopus subject areas

  • Pharmacology

Cite this

Estrogen-mediated up-regulation of the Ca-dependent constitutive nitric oxide synthase in the rat aorta and heart. / Morschl, E.; Bretus, Ildikó; Nemcsik, János; László, F.; Pávó, Imre.

In: Life Sciences, Vol. 68, No. 1, 24.11.2000, p. 49-55.

Research output: Contribution to journalArticle

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