Estrogen enhances expression of the complement C5a receptor and the C5a-agonist evoked calcium influx in hormone secreting neurons of the hypothalamus

Imre Farkas, Patricia Varju, Emese Szabo, E. Hrabovszky, Noriko Okada, Hidechika Okada, Z. Liposits

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

In the present study we examined presence of the complement C5a receptor (C5aR) in hypothalamic neurosecretory neurons of the rodent brain and effect of estrogen on C5aR expression. Whole cell patch clamp measurements revealed that magnocellular neurons in the supraoptic and paraventricular nuclei of hypothalamic slices of the rats responded to the C5aR-agonist PL37-MAP peptide with calcium ion current pulses. Gonadotropin-releasing hormone (GnRH) producing neurons in slices of the preoptic area of the mice also reacted to the peptide treatment with inward calcium current. PL37-MAP was able to evoke the inward ion current of GnRH neurons in slices from ovariectomized animals. The amplitude of the inward pulses became higher in slices obtained from 17β-estradiol (E2) substituted mice. Calcium imaging experiments demonstrated that PL37-MAP increased the intracellular calcium content in the culture of the GnRH-producing GT1-7 cell line in a concentration-dependent manner. Calcium imaging also showed that E2 pretreatment elevated the PL37-MAP evoked increase of the intracellular calcium content in the GT1-7 cells. The estrogen receptor blocker Faslodex in the medium prevented the E2-evoked increase of the PL37-MAP-triggered elevation of the intracellular calcium content in the GT1-7 cells demonstrating that the effect of E2 might be related to the presence of estrogen receptor. Real-time PCR experiments revealed that E2 increased the expression of C5aR mRNA in GT1-7 neurons, suggesting that an increased C5aR synthesis could be involved in the estrogenic modulation of calcium response. These data indicate that hypothalamic neuroendocrine neurons can integrate immune and neuroendocrine functions. Our results may serve a better understanding of the inflammatory and neurodegeneratory diseases of the hypothalamus and the related neuroendocrine and autonomic compensatory responses.

Original languageEnglish
Pages (from-to)846-856
Number of pages11
JournalNeurochemistry International
Volume52
Issue number4-5
DOIs
Publication statusPublished - Mar 2008

Fingerprint

Anaphylatoxin C5a Receptor
Complement C5a
Complement Receptors
Hypothalamus
Estrogens
Hormones
Calcium
Neurons
Gonadotropin-Releasing Hormone
Estrogen Receptors
Ions
Supraoptic Nucleus
Peptides
Preoptic Area
Paraventricular Hypothalamic Nucleus
Real-Time Polymerase Chain Reaction
Estradiol
Rodentia
Cell Line
Messenger RNA

Keywords

  • Complement C5a receptor
  • Estrogen
  • Hypothalamus
  • Neurosecretory neurons

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Cellular and Molecular Neuroscience

Cite this

Estrogen enhances expression of the complement C5a receptor and the C5a-agonist evoked calcium influx in hormone secreting neurons of the hypothalamus. / Farkas, Imre; Varju, Patricia; Szabo, Emese; Hrabovszky, E.; Okada, Noriko; Okada, Hidechika; Liposits, Z.

In: Neurochemistry International, Vol. 52, No. 4-5, 03.2008, p. 846-856.

Research output: Contribution to journalArticle

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