Estimation of pollen viability in einkorn (Triticum Monococcum ssp. Monococcum) accessions of different geographical origin

Research output: Contribution to journalArticle

Abstract

In the present experiment the pollen viability of einkorn accessions of different origin was tested using four vital dyes (TTC, Baker's procedure, MTT and FDA) to determine the potential of the dyes to differentiate fresh living pollen from pollen heated for 12 hours at 80°C (killed pollen). It was found that two of the four dyes previously employed to determine pollen viability also stained killed pollen in the case of several einkorn accessions, while FDA and MTT did not. It is thus suggested that the two latter should be used to test einkorn pollen viability, since they do not normally stain either killed or aborted pollen grains.

Original languageEnglish
Pages (from-to)405-411
Number of pages7
JournalActa Agronomica Hungarica
Volume51
Issue number4
DOIs
Publication statusPublished - 2003

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Triticum monococcum subsp. monococcum
Triticum monococcum
viability
pollen
dyes

Keywords

  • Drought tolerance
  • Einkorn
  • Pollen viability
  • Triticum monococcum ssp. monococcum
  • Vital staining

ASJC Scopus subject areas

  • Agronomy and Crop Science

Cite this

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title = "Estimation of pollen viability in einkorn (Triticum Monococcum ssp. Monococcum) accessions of different geographical origin",
abstract = "In the present experiment the pollen viability of einkorn accessions of different origin was tested using four vital dyes (TTC, Baker's procedure, MTT and FDA) to determine the potential of the dyes to differentiate fresh living pollen from pollen heated for 12 hours at 80°C (killed pollen). It was found that two of the four dyes previously employed to determine pollen viability also stained killed pollen in the case of several einkorn accessions, while FDA and MTT did not. It is thus suggested that the two latter should be used to test einkorn pollen viability, since they do not normally stain either killed or aborted pollen grains.",
keywords = "Drought tolerance, Einkorn, Pollen viability, Triticum monococcum ssp. monococcum, Vital staining",
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T1 - Estimation of pollen viability in einkorn (Triticum Monococcum ssp. Monococcum) accessions of different geographical origin

AU - Kovács, G.

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N2 - In the present experiment the pollen viability of einkorn accessions of different origin was tested using four vital dyes (TTC, Baker's procedure, MTT and FDA) to determine the potential of the dyes to differentiate fresh living pollen from pollen heated for 12 hours at 80°C (killed pollen). It was found that two of the four dyes previously employed to determine pollen viability also stained killed pollen in the case of several einkorn accessions, while FDA and MTT did not. It is thus suggested that the two latter should be used to test einkorn pollen viability, since they do not normally stain either killed or aborted pollen grains.

AB - In the present experiment the pollen viability of einkorn accessions of different origin was tested using four vital dyes (TTC, Baker's procedure, MTT and FDA) to determine the potential of the dyes to differentiate fresh living pollen from pollen heated for 12 hours at 80°C (killed pollen). It was found that two of the four dyes previously employed to determine pollen viability also stained killed pollen in the case of several einkorn accessions, while FDA and MTT did not. It is thus suggested that the two latter should be used to test einkorn pollen viability, since they do not normally stain either killed or aborted pollen grains.

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