Estimation of insulin antibodies with 125I insulin in vitro

I. Balazsi, M. Stutzel, I. Lang, L. Littmann

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Abstract

Insulin binding antibodies were investigated in venous blood by the method of Welborn et al. based on the principle laid down by Heding. 125I insulin (0.01 μUCi) was added to the diluted plasma and the mixture was incubated for 24 hr. The insulin insulin neutralizing antibody complex was precipitated with ethanol. The actual activity of the precipitate was calculated as a percentage of total activity. Among the 107 diabetic patients tested, the sera of those treated with insulin for less than 6 mth showed a moderate increase in 125I insulin binding capacity (17-30%). The sera of patients treated for longer than 6 mth gave a higher binding rate (34-45%). Two sera of insulin resistant diabetics showed a marked 125I insulin binding above 60%. No false positive or negative results were observed. The method is recommended in laboratories having 125I insulin preparations with high specific activity for the estimation of insulin binding antibody levels of insulin treated and insulin resistant diabetics.

Original languageEnglish
Pages (from-to)95-97
Number of pages3
JournalREV.ROUM.MED.INTERNE
Volume11
Issue number1
Publication statusPublished - Jan 1 1974

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ASJC Scopus subject areas

  • Medicine(all)

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Balazsi, I., Stutzel, M., Lang, I., & Littmann, L. (1974). Estimation of insulin antibodies with 125I insulin in vitro. REV.ROUM.MED.INTERNE, 11(1), 95-97.