Three minor impurities were detected and identified in estradiol bulk material. Estradiol-17-acetate was identified by HPLC and TLC retention matching with an authentic sample. 9(11)-Dehydroestradiol was identified by means of HPLC/diode-array UV spectroscopy. On the basis of its mass spectrum and HPLC/diode-array UV spectrum the third impurity was the 4-chloro derivative of estradiol. The identification of the impurities was confirmed by synthesizing the proposed structures and retention matching of the impurities and the synthesized materials.
|Number of pages||4|
|Journal||Analytical methods & instrumentation|
|Publication status||Published - Jan 1 1995|
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