Ester composition of carnitine in the perfusate of liver and in the plasma of donor rats

A. Sandor, G. Kispál, B. Melegh, I. Alkonyi

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

When the carnitine pool of fed rats was labelled with tritium, in non-recirculating perfusate of their liver 44% of acid-soluble 3H activity was identified as free carnitine and 47% as short-chain acylcarnitine. Of the latter component acetylcarnitine accounted for 30% and propionylcarnitine for 10% of total acid-soluble. In plasma the contribution of short-chain acylcarnitines to total carnitine in fed, fasted and diabetic rats was 16.5%, 43.1% and 48.0%, respectively. Recirculating perfusion of livers from the same animals revealed that livers from fed rats released short-chain acylcarnitines as much as 56.2% of total and this proportion did not increase further in the other two groups. At the same time, ketone bodies in the perfusate increased gradually in the fed, fasted and diabetic group, paralleling the plasma ketone levels. Although liver supplies the organism with carnitine the increment of plasma short-chain acylcarnitines seen in ketosis is not a result of some extra output by the liver.

Original languageEnglish
Pages (from-to)443-445
Number of pages3
JournalEuropean Journal of Biochemistry
Volume170
Issue number1-2
Publication statusPublished - 1988

Fingerprint

Carnitine
Liver
Rats
Esters
Plasmas
Chemical analysis
propionylcarnitine
Acetylcarnitine
Ketone Bodies
Ketosis
Acids
Tritium
Ketones
Animals
Perfusion
acylcarnitine

ASJC Scopus subject areas

  • Biochemistry

Cite this

Ester composition of carnitine in the perfusate of liver and in the plasma of donor rats. / Sandor, A.; Kispál, G.; Melegh, B.; Alkonyi, I.

In: European Journal of Biochemistry, Vol. 170, No. 1-2, 1988, p. 443-445.

Research output: Contribution to journalArticle

@article{0f7cee7fe7994f8aa8bd37e8cabc005b,
title = "Ester composition of carnitine in the perfusate of liver and in the plasma of donor rats",
abstract = "When the carnitine pool of fed rats was labelled with tritium, in non-recirculating perfusate of their liver 44{\%} of acid-soluble 3H activity was identified as free carnitine and 47{\%} as short-chain acylcarnitine. Of the latter component acetylcarnitine accounted for 30{\%} and propionylcarnitine for 10{\%} of total acid-soluble. In plasma the contribution of short-chain acylcarnitines to total carnitine in fed, fasted and diabetic rats was 16.5{\%}, 43.1{\%} and 48.0{\%}, respectively. Recirculating perfusion of livers from the same animals revealed that livers from fed rats released short-chain acylcarnitines as much as 56.2{\%} of total and this proportion did not increase further in the other two groups. At the same time, ketone bodies in the perfusate increased gradually in the fed, fasted and diabetic group, paralleling the plasma ketone levels. Although liver supplies the organism with carnitine the increment of plasma short-chain acylcarnitines seen in ketosis is not a result of some extra output by the liver.",
author = "A. Sandor and G. Kisp{\'a}l and B. Melegh and I. Alkonyi",
year = "1988",
language = "English",
volume = "170",
pages = "443--445",
journal = "FEBS Journal",
issn = "1742-464X",
publisher = "Wiley-Blackwell",
number = "1-2",

}

TY - JOUR

T1 - Ester composition of carnitine in the perfusate of liver and in the plasma of donor rats

AU - Sandor, A.

AU - Kispál, G.

AU - Melegh, B.

AU - Alkonyi, I.

PY - 1988

Y1 - 1988

N2 - When the carnitine pool of fed rats was labelled with tritium, in non-recirculating perfusate of their liver 44% of acid-soluble 3H activity was identified as free carnitine and 47% as short-chain acylcarnitine. Of the latter component acetylcarnitine accounted for 30% and propionylcarnitine for 10% of total acid-soluble. In plasma the contribution of short-chain acylcarnitines to total carnitine in fed, fasted and diabetic rats was 16.5%, 43.1% and 48.0%, respectively. Recirculating perfusion of livers from the same animals revealed that livers from fed rats released short-chain acylcarnitines as much as 56.2% of total and this proportion did not increase further in the other two groups. At the same time, ketone bodies in the perfusate increased gradually in the fed, fasted and diabetic group, paralleling the plasma ketone levels. Although liver supplies the organism with carnitine the increment of plasma short-chain acylcarnitines seen in ketosis is not a result of some extra output by the liver.

AB - When the carnitine pool of fed rats was labelled with tritium, in non-recirculating perfusate of their liver 44% of acid-soluble 3H activity was identified as free carnitine and 47% as short-chain acylcarnitine. Of the latter component acetylcarnitine accounted for 30% and propionylcarnitine for 10% of total acid-soluble. In plasma the contribution of short-chain acylcarnitines to total carnitine in fed, fasted and diabetic rats was 16.5%, 43.1% and 48.0%, respectively. Recirculating perfusion of livers from the same animals revealed that livers from fed rats released short-chain acylcarnitines as much as 56.2% of total and this proportion did not increase further in the other two groups. At the same time, ketone bodies in the perfusate increased gradually in the fed, fasted and diabetic group, paralleling the plasma ketone levels. Although liver supplies the organism with carnitine the increment of plasma short-chain acylcarnitines seen in ketosis is not a result of some extra output by the liver.

UR - http://www.scopus.com/inward/record.url?scp=0023870573&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023870573&partnerID=8YFLogxK

M3 - Article

VL - 170

SP - 443

EP - 445

JO - FEBS Journal

JF - FEBS Journal

SN - 1742-464X

IS - 1-2

ER -