Establishment of induced pluripotent stem cell (iPSC) line from a 75-year old patient with late onset Alzheimer's disease (LOAD)

Zsuzsanna Táncos, Eszter Varga, Eszter Kovács, A. Dinnyés, Julianna Kobolák

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Peripheral blood mononuclear cells (PBMCs) were collected from a clinically characterised 75-year old woman with late onset Alzheimer's disease (LOAD). The PMBCs were reprogrammed with the human OSKM transcription factors using the Sendai-virus delivery system. The transgene-free iPSC showed pluripotency verified by immunocytochemistry for pluripotency markers and differentiated spontaneously towards the 3 germ layers in vitro. Furthermore, the iPSC line showed normal karyotype. Our model might offer a good platform to further study the pathomechanism of sporadic AD, to identify early biomarkers and also for drug testing and gene therapy studies.

Original languageEnglish
Pages (from-to)81-83
Number of pages3
JournalStem Cell Research
Volume17
Issue number1
DOIs
Publication statusPublished - Jul 1 2016

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Induced Pluripotent Stem Cells
Alzheimer Disease
Sendai virus
Cell Line
Germ Layers
Transgenes
Karyotype
Genetic Therapy
Blood Cells
Transcription Factors
Biomarkers
Immunohistochemistry
Drug Therapy
In Vitro Techniques

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Medicine(all)

Cite this

Establishment of induced pluripotent stem cell (iPSC) line from a 75-year old patient with late onset Alzheimer's disease (LOAD). / Táncos, Zsuzsanna; Varga, Eszter; Kovács, Eszter; Dinnyés, A.; Kobolák, Julianna.

In: Stem Cell Research, Vol. 17, No. 1, 01.07.2016, p. 81-83.

Research output: Contribution to journalArticle

Táncos, Zsuzsanna ; Varga, Eszter ; Kovács, Eszter ; Dinnyés, A. ; Kobolák, Julianna. / Establishment of induced pluripotent stem cell (iPSC) line from a 75-year old patient with late onset Alzheimer's disease (LOAD). In: Stem Cell Research. 2016 ; Vol. 17, No. 1. pp. 81-83.
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