Establishment of induced pluripotent stem cell (iPSC) line from a 63-year old patient with late onset Alzheimer's disease (LOAD)

Abinaya Chandrasekaran, Eszter Varga, Csilla Nemes, Zsuzsanna Táncos, Julianna Kobolák, A. Dinnyés

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Peripheral blood mononuclear cells (PBMCs) were collected from a clinically characterised 63-year old woman with late onset Alzheimer's disease (LOAD). The PMBCs were reprogrammed with the human OSKM transcription factors using the Sendai-virus delivery system. The transgene-free iPSC showed pluripotency verified by immunocytochemistry for pluripotency markers and differentiated spontaneously towards the 3 germ layers in vitro. Furthermore, the iPSC line showed normal karyotype. Our model might offer a good platform to further study the pathomechanism of sporadic AD, to identify early biomarkers and also for drug testing and gene therapy studies.

Original languageEnglish
Pages (from-to)78-80
Number of pages3
JournalStem Cell Research
Volume17
Issue number1
DOIs
Publication statusPublished - Jul 1 2016

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ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Medicine(all)

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