Establishment of grapevine embryogenic liquid culture and induced somatic embryogenesis

I. Forgács, B. Suller, A. Zok, A. Pedryc, R. Oláh, T. Deák, Gy D. Bisztray, E. Szegedi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Somatic embryogenesis is a process in which somatic cells can be used to regenerate embryos and whole plants. Embryogenic cell suspensions of 'Chardonnay' and 'Richter 110' grapevine cultivars were established in 8 different liquid media, cultures had been later maintained in solid media. By the end of the sixth week, we achieved 7-fold increase of fresh weight in the case of the 'Chardonnay' cultivar, using MSM1 and CP2 liquid media. Four different pH values of hormone-free MSM1 media were tested in our experiments (pH 5.8, pH 4.6, pH 4.3 were adjusted before autoclaving; or pH 4.3 after autoclaving). In the case of pH 5.8, we measured a 16-fold increase of fresh weight of the 'Richter110' cultivar. Various culture densities (0.25-4 mg mL-1) were tested, and found that the one with 2 mg mL-1 density was the most efficient. Somatic embryogenesis proved to be significantly more efficient when the medium was supplemented with 0.5 g L-1 MES buffer [2-(N-morpholino) ethanesulfonic acid]. In this case, however, the embryos regenerated developed highly elongated hypocotyls, which inhibited the germination of the embryos as well as the successful regeneration of plants. In a half-strength MSM1 medium with a cell density of 1 mg mL-1, the cultures were composed of well synchronised globular stage embryos. In this setup, however, further differentiation was reversibly inhibited. This inhibition can be resolved by a second dilution of the cultures (to 1 mg mL-1 cell density). When hormone-free MSM1 medium was used after the second dilution, which was enriched with 1 g L-1 activated charcoal, synchronised and well developed cotyledonary stage embryos were differentiated in the liquid cultures by the sixth week.

Original languageEnglish
Title of host publication9th International Symposium on Grapevine Physiology and Biotechnology
PublisherInternational Society for Horticultural Science
Pages113-118
Number of pages6
ISBN (Electronic)9789462611535
DOIs
Publication statusPublished - Apr 26 2017

Publication series

NameActa Horticulturae
Volume1157
ISSN (Print)0567-7572

Fingerprint

somatic embryogenesis
embryo (plant)
liquids
autoclaving
cultivars
hormones
activated carbon
somatic cells
cell suspension culture
hypocotyls
buffers
culture media
cells
germination
acids

Keywords

  • 2-naphtoxyacetic acid
  • Activated charcoal
  • Cell suspension
  • Maltose
  • Plant regeneration
  • Vitis vinifera

ASJC Scopus subject areas

  • Horticulture

Cite this

Forgács, I., Suller, B., Zok, A., Pedryc, A., Oláh, R., Deák, T., ... Szegedi, E. (2017). Establishment of grapevine embryogenic liquid culture and induced somatic embryogenesis. In 9th International Symposium on Grapevine Physiology and Biotechnology (pp. 113-118). (Acta Horticulturae; Vol. 1157). International Society for Horticultural Science. https://doi.org/10.17660/ActaHortic.2017.1157.18

Establishment of grapevine embryogenic liquid culture and induced somatic embryogenesis. / Forgács, I.; Suller, B.; Zok, A.; Pedryc, A.; Oláh, R.; Deák, T.; Bisztray, Gy D.; Szegedi, E.

9th International Symposium on Grapevine Physiology and Biotechnology. International Society for Horticultural Science, 2017. p. 113-118 (Acta Horticulturae; Vol. 1157).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Forgács, I, Suller, B, Zok, A, Pedryc, A, Oláh, R, Deák, T, Bisztray, GD & Szegedi, E 2017, Establishment of grapevine embryogenic liquid culture and induced somatic embryogenesis. in 9th International Symposium on Grapevine Physiology and Biotechnology. Acta Horticulturae, vol. 1157, International Society for Horticultural Science, pp. 113-118. https://doi.org/10.17660/ActaHortic.2017.1157.18
Forgács I, Suller B, Zok A, Pedryc A, Oláh R, Deák T et al. Establishment of grapevine embryogenic liquid culture and induced somatic embryogenesis. In 9th International Symposium on Grapevine Physiology and Biotechnology. International Society for Horticultural Science. 2017. p. 113-118. (Acta Horticulturae). https://doi.org/10.17660/ActaHortic.2017.1157.18
Forgács, I. ; Suller, B. ; Zok, A. ; Pedryc, A. ; Oláh, R. ; Deák, T. ; Bisztray, Gy D. ; Szegedi, E. / Establishment of grapevine embryogenic liquid culture and induced somatic embryogenesis. 9th International Symposium on Grapevine Physiology and Biotechnology. International Society for Horticultural Science, 2017. pp. 113-118 (Acta Horticulturae).
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