Establishment of an induced pluripotent stem cell (iPSC) line from a 9-year old male with autism spectrum disorder (ASD)

Eszter Varga, Csilla Nemes, István Bock, Zsuzsanna Táncos, Sára Berzsenyi, György Lévay, Viktor Román, Julianna Kobolák, A. Dinnyés

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Peripheral blood mononuclear cells (PBMCs) were collected from a clinically characterized patient with autism spectrum disorder (ASD). The PMBCs were reprogrammed with the human OSKM transcription factors using the Sendai-virus delivery system. The pluripotency of transgene-free iPSCs was verified by immunocytochemistry for pluripotency markers and by spontaneous in vitro differentiation towards the 3 germ layers. Furthermore, the iPSC line showed normal karyotype. Our model might offer a good platform to study the pathomechanism of ASD, also for drug testing, early biomarker discovery and gene therapy studies.

Original languageEnglish
Pages (from-to)19-22
Number of pages4
JournalStem Cell Research
Volume21
DOIs
Publication statusPublished - May 1 2017

ASJC Scopus subject areas

  • Medicine(all)
  • Developmental Biology
  • Cell Biology

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