Establishment of an induced pluripotent stem cell (iPSC) line from a 9-year old male with autism spectrum disorder (ASD)

Eszter Varga, Csilla Nemes, István Bock, Zsuzsanna Táncos, Sára Berzsenyi, György Lévay, Viktor Román, Julianna Kobolák, A. Dinnyés

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Peripheral blood mononuclear cells (PBMCs) were collected from a clinically characterized patient with autism spectrum disorder (ASD). The PMBCs were reprogrammed with the human OSKM transcription factors using the Sendai-virus delivery system. The pluripotency of transgene-free iPSCs was verified by immunocytochemistry for pluripotency markers and by spontaneous in vitro differentiation towards the 3 germ layers. Furthermore, the iPSC line showed normal karyotype. Our model might offer a good platform to study the pathomechanism of ASD, also for drug testing, early biomarker discovery and gene therapy studies.

Original languageEnglish
Pages (from-to)19-22
Number of pages4
JournalStem Cell Research
Volume21
DOIs
Publication statusPublished - May 1 2017

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Induced Pluripotent Stem Cells
Sendai virus
Cell Line
Germ Layers
Transgenes
Karyotype
Genetic Therapy
Blood Cells
Transcription Factors
Biomarkers
Immunohistochemistry
Pharmaceutical Preparations
Autism Spectrum Disorder
In Vitro Techniques

ASJC Scopus subject areas

  • Medicine(all)
  • Developmental Biology
  • Cell Biology

Cite this

Establishment of an induced pluripotent stem cell (iPSC) line from a 9-year old male with autism spectrum disorder (ASD). / Varga, Eszter; Nemes, Csilla; Bock, István; Táncos, Zsuzsanna; Berzsenyi, Sára; Lévay, György; Román, Viktor; Kobolák, Julianna; Dinnyés, A.

In: Stem Cell Research, Vol. 21, 01.05.2017, p. 19-22.

Research output: Contribution to journalArticle

Varga, Eszter ; Nemes, Csilla ; Bock, István ; Táncos, Zsuzsanna ; Berzsenyi, Sára ; Lévay, György ; Román, Viktor ; Kobolák, Julianna ; Dinnyés, A. / Establishment of an induced pluripotent stem cell (iPSC) line from a 9-year old male with autism spectrum disorder (ASD). In: Stem Cell Research. 2017 ; Vol. 21. pp. 19-22.
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