Establishment of an in vivo ARGONAUTE reporter system in plants

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

RNA silencing is not only an evolutionarily conserved gene regulatory mechanism, but in plants also serves as the basis for robust adaptive antiviral immune responses. ARGONAUTE (AGO) proteins form the catalytic cores of the RNA-guided ribonuclease complexes, which play a central role in RNA silencing. Here we describe an in vivo assay system for analyzing the activities of AGO proteins in the virological model plant Nicotiana benthamiana.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages73-91
Number of pages19
DOIs
Publication statusPublished - Jan 1 2017

Publication series

NameMethods in Molecular Biology
Volume1640
ISSN (Print)1064-3745

Keywords

  • Agroinfiltratio n
  • ARGONAUTE
  • Nicotiana benthamiana
  • RNA silencing
  • Transient reporter system

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Fátyol, K., & Burgyán, J. (2017). Establishment of an in vivo ARGONAUTE reporter system in plants. In Methods in Molecular Biology (pp. 73-91). (Methods in Molecular Biology; Vol. 1640). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7165-7_5