Establishment and characterization of a new transplantable pancreatic cancer xenograft (PZX-5) in immunosuppressed mice

A. Zalatnai, József Bocsi, Ferenc Timár, István Babó

Research output: Contribution to journalArticle

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Abstract

Conclusion. A new, stable, transplantable human pancreatic cancer xenograft (PZX-5) model has been established in CBA immunosuppressed mice. Background. Numerous human pancreatic carcinomas have been successfully transplanted into athymic nude mice. However, artificially immunosuppressed animals have rarely been used as recipients. Because this model system proved to be reliable for hosting many human malignancies at our institute, successive xenotransplantations of a ductal adenocarcinoma have been carried out. Method. Immunosuppression of CBA/CA mice was achieved by thymectomy, whole-body irradiation and bone-marrow reconstruction. Tumor fragments were subcutaneously implanted from a well/moderately differentiated ductal pancreatic adenocarcinoma and serially transplanted for more than 20 mo. The xenografted tumors were characterized using morphological, immunohistochemical, biochemical, and flow cytometric methods. Results. During the serial transplantations, the neoplasm maintained its original morphological-pathobiological characteristics. It produced a large amount of mucin and expressed carcinoembryonic antigen (CEA). Neither the mitotic activity nor the degree of differentiation was altered, and CEA was permanently detected. Flow cytometric DNA analysis revealed an aneuploid pattern (DNA index 1.45 ± 0.03), which has remained within the same range during xenograftings. The doubling time in an in vitro system proved to be 18 h. The human character has been well preserved even 9 mo posttransplantation, as was evidenced by LDH-isoenzyme electrophoresis. The results indicate that the thymectomized - whole-body irradiated - bone-marrow reconstructed immunosuppressed mice are also appropriate hosts for pancreatic cancer xenografts.

Original languageEnglish
Pages (from-to)51-62
Number of pages12
JournalInternational Journal of Pancreatology
Volume23
Issue number1
Publication statusPublished - 1998

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Pancreatic Neoplasms
Heterografts
Inbred CBA Mouse
Heterologous Transplantation
Carcinoembryonic Antigen
Neoplasm Transplantation
Nude Mice
Adenocarcinoma
Bone Marrow
Neoplasms
Thymectomy
Whole-Body Irradiation
DNA
Aneuploidy
Mucins
Immunosuppression
Isoenzymes
Electrophoresis

Keywords

  • Immunosuppressed mice
  • Pancreatic carcinoma
  • PZX-5

ASJC Scopus subject areas

  • Gastroenterology
  • Endocrinology
  • Oncology

Cite this

Establishment and characterization of a new transplantable pancreatic cancer xenograft (PZX-5) in immunosuppressed mice. / Zalatnai, A.; Bocsi, József; Timár, Ferenc; Babó, István.

In: International Journal of Pancreatology, Vol. 23, No. 1, 1998, p. 51-62.

Research output: Contribution to journalArticle

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N2 - Conclusion. A new, stable, transplantable human pancreatic cancer xenograft (PZX-5) model has been established in CBA immunosuppressed mice. Background. Numerous human pancreatic carcinomas have been successfully transplanted into athymic nude mice. However, artificially immunosuppressed animals have rarely been used as recipients. Because this model system proved to be reliable for hosting many human malignancies at our institute, successive xenotransplantations of a ductal adenocarcinoma have been carried out. Method. Immunosuppression of CBA/CA mice was achieved by thymectomy, whole-body irradiation and bone-marrow reconstruction. Tumor fragments were subcutaneously implanted from a well/moderately differentiated ductal pancreatic adenocarcinoma and serially transplanted for more than 20 mo. The xenografted tumors were characterized using morphological, immunohistochemical, biochemical, and flow cytometric methods. Results. During the serial transplantations, the neoplasm maintained its original morphological-pathobiological characteristics. It produced a large amount of mucin and expressed carcinoembryonic antigen (CEA). Neither the mitotic activity nor the degree of differentiation was altered, and CEA was permanently detected. Flow cytometric DNA analysis revealed an aneuploid pattern (DNA index 1.45 ± 0.03), which has remained within the same range during xenograftings. The doubling time in an in vitro system proved to be 18 h. The human character has been well preserved even 9 mo posttransplantation, as was evidenced by LDH-isoenzyme electrophoresis. The results indicate that the thymectomized - whole-body irradiated - bone-marrow reconstructed immunosuppressed mice are also appropriate hosts for pancreatic cancer xenografts.

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