Esophageal replacement using cryopreserved tracheal graft

Á Juhász, A. Szilágyi, I. Mikó, I. Altorjay, G. Kecskés, Áron Altorjay

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Esophageal replacement using artificial material is not a new concept. Prior experiences with prostheses, allografts and composite grafts have not proved particularly successful. The aim of this study is to investigate whether cryopreserved animal trachea is suitable for the replacement of the esophagus. In 12 beagle dogs a 6-cm-long segment of the cervical esophagus was removed, and was replaced with cryopreserved trachea, which had been stored for 21 days on -86°C. The proximal and distal ends of the esophagus were joined together with the graft by simple continuous suture (Biosyn® 3/0) and covered with the sternohyoid flap. Postoperatively 16 hematological parameters were measured. The dogs were planned to be euthanized at random on days 28, 42 and 56 after the operation. Tests for air leak were performed and the inner diameter of the graft was measured to detect shrinkage. The microscopic structure of the graft was analyzed using haematoxylin and eosin staining. There was no indication of insufficiency. Based on the air leak test the sutures withheld properly. The inner diameter of the graft narrowed from an average 19 mm (± 1 mm) to 15.8 mm (± 0.6 mm). In length, the graft shortened from an average 60 mm to 47 mm (± 3 mm). No feeding difficulty was observed. In two cases wound suppuration was found involving only the cutaneous and subcutaneous layers. Concerning the laboratory parameters, only the fibrinogen level and white blood cell count showed temporary although significant changes. Histology findings on the 56th day showed absolute integration of the trachea with the esophagus, with disintegration of the tracheal cartilages. Cryopreserved trachea seems to be suitable for the replacement of a 5-6-cm-long esophageal segment.

Original languageEnglish
Pages (from-to)468-472
Number of pages5
JournalDiseases of the Esophagus
Volume21
Issue number5
DOIs
Publication statusPublished - Aug 2008

Fingerprint

Trachea
Transplants
Esophagus
Air
Dogs
Suppuration
Hematoxylin
Eosine Yellowish-(YS)
Leukocyte Count
Fibrinogen
Sutures
Prostheses and Implants
Cartilage
Allografts
Histology
Staining and Labeling
Skin
Wounds and Injuries

Keywords

  • Allotransplantation
  • Cryopreservation
  • Esophagus replacement
  • Trachea segment

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Esophageal replacement using cryopreserved tracheal graft. / Juhász, Á; Szilágyi, A.; Mikó, I.; Altorjay, I.; Kecskés, G.; Altorjay, Áron.

In: Diseases of the Esophagus, Vol. 21, No. 5, 08.2008, p. 468-472.

Research output: Contribution to journalArticle

Juhász, Á ; Szilágyi, A. ; Mikó, I. ; Altorjay, I. ; Kecskés, G. ; Altorjay, Áron. / Esophageal replacement using cryopreserved tracheal graft. In: Diseases of the Esophagus. 2008 ; Vol. 21, No. 5. pp. 468-472.
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