Esophageal ATP synthase and keratinocyte growth factor gene expression changes after acid and bile-induced mucosal damage

K. Szentpáli, A. Paszt, G. Lazar, M. Széll, L. Kemény, A. Dobozy, J. Kaszaki, L. Tiszlavicz, L. Iván, M. Borós

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2 Citations (Scopus)

Abstract

Objective and design: Intramural gene expression changes may be critically involved in tissue damage, defense and repair after esophageal regurgitation. The aims were to characterize the consequences of short-term exposure to luminal bile, acid, or bile mixed with acid on the β-ATPase, keratinocyte growth factor 1 (KGF-1) and KGF receptor (KGF-R) expressions within the mucosa and the muscle layer in a large animal model. Materials and subjects: Esophageal segments of anesthetized dogs were exposed to saline (n = 3), diluted canine bile (n = 6), hydrochloric acid (n = 5) or bile + hydrochloric acid (n = 5), and tissue biopsies were taken at the end of the 180-min observation period. Semiquantitative reverse transcriptase polymerase chain reactions were carried out and the degree of histological damage was evaluated on the 0-16-grade Geisinger scoring scale. Results: Acid exposure was followed by a significant decrease in the level of β-ATPase expression in the mucosa, and parallel increases in KGF-1 and KGF-R expression. Corresponding changes in the muscle layer were not significant. Bile alone evoked more severe tissue damage, with significantly decreased β-ATPase levels in both the mucosa and the muscle, whereas the KGF-1 expression did not change significantly. The bile + acid treatment induced an intermediate state, with significant β-ATPase transcription level decreases in both layers, while the mucosal KGF-1 expression was lower than that following acid treatment alone. Conclusions: The acid-induced transcriptional level down-regulation of mucosal β-ATPase gene expression in the smooth muscle layer was exacerbated by bile, but the concomitant KGF and KGF-R gene expression changes may indicate the start of a consecutive repair process.

Original languageEnglish
Pages (from-to)152-157
Number of pages6
JournalInflammation Research
Volume54
Issue number4
DOIs
Publication statusPublished - Apr 2005

Fingerprint

Fibroblast Growth Factor 7
Bile Acids and Salts
Gene expression
Adenosine Triphosphatases
Bile
Adenosine Triphosphate
Muscle
Gene Expression
Mucous Membrane
Acids
Hydrochloric Acid
Tissue
Muscles
Repair
Biopsy
Polymerase chain reaction
RNA-Directed DNA Polymerase
Transcription
Reverse Transcriptase Polymerase Chain Reaction
Smooth Muscle

Keywords

  • βATP synthase
  • Acute regurgitation
  • Experimental esophagitis
  • Gene expression
  • Keratinocyte growth factor
  • Mucosa

ASJC Scopus subject areas

  • Toxicology
  • Pharmacology (medical)
  • Immunology
  • Cell Biology

Cite this

@article{283c5c5cf02644439884906dfa91c5d2,
title = "Esophageal ATP synthase and keratinocyte growth factor gene expression changes after acid and bile-induced mucosal damage",
abstract = "Objective and design: Intramural gene expression changes may be critically involved in tissue damage, defense and repair after esophageal regurgitation. The aims were to characterize the consequences of short-term exposure to luminal bile, acid, or bile mixed with acid on the β-ATPase, keratinocyte growth factor 1 (KGF-1) and KGF receptor (KGF-R) expressions within the mucosa and the muscle layer in a large animal model. Materials and subjects: Esophageal segments of anesthetized dogs were exposed to saline (n = 3), diluted canine bile (n = 6), hydrochloric acid (n = 5) or bile + hydrochloric acid (n = 5), and tissue biopsies were taken at the end of the 180-min observation period. Semiquantitative reverse transcriptase polymerase chain reactions were carried out and the degree of histological damage was evaluated on the 0-16-grade Geisinger scoring scale. Results: Acid exposure was followed by a significant decrease in the level of β-ATPase expression in the mucosa, and parallel increases in KGF-1 and KGF-R expression. Corresponding changes in the muscle layer were not significant. Bile alone evoked more severe tissue damage, with significantly decreased β-ATPase levels in both the mucosa and the muscle, whereas the KGF-1 expression did not change significantly. The bile + acid treatment induced an intermediate state, with significant β-ATPase transcription level decreases in both layers, while the mucosal KGF-1 expression was lower than that following acid treatment alone. Conclusions: The acid-induced transcriptional level down-regulation of mucosal β-ATPase gene expression in the smooth muscle layer was exacerbated by bile, but the concomitant KGF and KGF-R gene expression changes may indicate the start of a consecutive repair process.",
keywords = "βATP synthase, Acute regurgitation, Experimental esophagitis, Gene expression, Keratinocyte growth factor, Mucosa",
author = "K. Szentp{\'a}li and A. Paszt and G. Lazar and M. Sz{\'e}ll and L. Kem{\'e}ny and A. Dobozy and J. Kaszaki and L. Tiszlavicz and L. Iv{\'a}n and M. Bor{\'o}s",
year = "2005",
month = "4",
doi = "10.1007/s00011-004-1336-3",
language = "English",
volume = "54",
pages = "152--157",
journal = "Inflammation Research",
issn = "1023-3830",
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TY - JOUR

T1 - Esophageal ATP synthase and keratinocyte growth factor gene expression changes after acid and bile-induced mucosal damage

AU - Szentpáli, K.

AU - Paszt, A.

AU - Lazar, G.

AU - Széll, M.

AU - Kemény, L.

AU - Dobozy, A.

AU - Kaszaki, J.

AU - Tiszlavicz, L.

AU - Iván, L.

AU - Borós, M.

PY - 2005/4

Y1 - 2005/4

N2 - Objective and design: Intramural gene expression changes may be critically involved in tissue damage, defense and repair after esophageal regurgitation. The aims were to characterize the consequences of short-term exposure to luminal bile, acid, or bile mixed with acid on the β-ATPase, keratinocyte growth factor 1 (KGF-1) and KGF receptor (KGF-R) expressions within the mucosa and the muscle layer in a large animal model. Materials and subjects: Esophageal segments of anesthetized dogs were exposed to saline (n = 3), diluted canine bile (n = 6), hydrochloric acid (n = 5) or bile + hydrochloric acid (n = 5), and tissue biopsies were taken at the end of the 180-min observation period. Semiquantitative reverse transcriptase polymerase chain reactions were carried out and the degree of histological damage was evaluated on the 0-16-grade Geisinger scoring scale. Results: Acid exposure was followed by a significant decrease in the level of β-ATPase expression in the mucosa, and parallel increases in KGF-1 and KGF-R expression. Corresponding changes in the muscle layer were not significant. Bile alone evoked more severe tissue damage, with significantly decreased β-ATPase levels in both the mucosa and the muscle, whereas the KGF-1 expression did not change significantly. The bile + acid treatment induced an intermediate state, with significant β-ATPase transcription level decreases in both layers, while the mucosal KGF-1 expression was lower than that following acid treatment alone. Conclusions: The acid-induced transcriptional level down-regulation of mucosal β-ATPase gene expression in the smooth muscle layer was exacerbated by bile, but the concomitant KGF and KGF-R gene expression changes may indicate the start of a consecutive repair process.

AB - Objective and design: Intramural gene expression changes may be critically involved in tissue damage, defense and repair after esophageal regurgitation. The aims were to characterize the consequences of short-term exposure to luminal bile, acid, or bile mixed with acid on the β-ATPase, keratinocyte growth factor 1 (KGF-1) and KGF receptor (KGF-R) expressions within the mucosa and the muscle layer in a large animal model. Materials and subjects: Esophageal segments of anesthetized dogs were exposed to saline (n = 3), diluted canine bile (n = 6), hydrochloric acid (n = 5) or bile + hydrochloric acid (n = 5), and tissue biopsies were taken at the end of the 180-min observation period. Semiquantitative reverse transcriptase polymerase chain reactions were carried out and the degree of histological damage was evaluated on the 0-16-grade Geisinger scoring scale. Results: Acid exposure was followed by a significant decrease in the level of β-ATPase expression in the mucosa, and parallel increases in KGF-1 and KGF-R expression. Corresponding changes in the muscle layer were not significant. Bile alone evoked more severe tissue damage, with significantly decreased β-ATPase levels in both the mucosa and the muscle, whereas the KGF-1 expression did not change significantly. The bile + acid treatment induced an intermediate state, with significant β-ATPase transcription level decreases in both layers, while the mucosal KGF-1 expression was lower than that following acid treatment alone. Conclusions: The acid-induced transcriptional level down-regulation of mucosal β-ATPase gene expression in the smooth muscle layer was exacerbated by bile, but the concomitant KGF and KGF-R gene expression changes may indicate the start of a consecutive repair process.

KW - βATP synthase

KW - Acute regurgitation

KW - Experimental esophagitis

KW - Gene expression

KW - Keratinocyte growth factor

KW - Mucosa

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U2 - 10.1007/s00011-004-1336-3

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JF - Inflammation Research

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