Erythrocytes From Patients With Type 2 Diabetes Induce Endothelial Dysfunction Via Arginase I

Zhichao Zhou, Ali Mahdi, Yahor Tratsiakovich, Szabolcs Zahorán, Oskar Kövamees, Filip Nordin, Arturo Eduardo Uribe Gonzalez, Michael Alvarsson, Claes Göran Östenson, Daniel C. Andersson, Ulf Hedin, E. Hermesz, Jon O. Lundberg, Jiangning Yang, John Pernow

Research output: Contribution to journalArticle

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Abstract

Background: Cardiovascular complications are major clinical problems in type 2 diabetes mellitus (T2DM). The authors previously demonstrated a crucial role of red blood cells (RBCs) in control of cardiac function through arginase-dependent regulation of nitric oxide export from RBCs. There is alteration of RBC function, as well as an increase in arginase activity, in T2DM. Objectives: The authors hypothesized that RBCs from patients with T2DM induce endothelial dysfunction by up-regulation of arginase. Methods: RBCs were isolated from patients with T2DM and age-matched healthy subjects and were incubated with rat aortas or human internal mammary arteries from nondiabetic patients for vascular reactivity and biochemical studies. Results: Arginase activity and arginase I protein expression were elevated in RBCs from patients with T2DM (T2DM RBCs) through an effect induced by reactive oxygen species (ROS). Co-incubation of arterial segments with T2DM RBCs, but not RBCs from age-matched healthy subjects, significantly impaired endothelial function but not smooth muscle cell function in both healthy rat aortas and human internal mammary arteries. Endothelial dysfunction induced by T2DM RBCs was prevented by inhibition of arginase and ROS both at the RBC and vascular levels. T2DM RBCs induced increased vascular arginase I expression and activity through an ROS-dependent mechanism. Conclusions: This study demonstrates a novel mechanism behind endothelial dysfunction in T2DM that is induced by RBC arginase I and ROS. Targeting arginase I in RBCs may serve as a novel therapeutic tool for the treatment of endothelial dysfunction in T2DM.

Original languageEnglish
Pages (from-to)769-780
Number of pages12
JournalJournal of the American College of Cardiology
Volume72
Issue number7
DOIs
Publication statusPublished - Aug 14 2018

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Arginase
Type 2 Diabetes Mellitus
Erythrocytes
Reactive Oxygen Species
Blood Vessels
Mammary Arteries
Aorta
Healthy Volunteers

Keywords

  • arginase
  • endothelial dysfunction
  • reactive oxygen species
  • red blood cell
  • type 2 diabetes
  • vascular complication

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Zhou, Z., Mahdi, A., Tratsiakovich, Y., Zahorán, S., Kövamees, O., Nordin, F., ... Pernow, J. (2018). Erythrocytes From Patients With Type 2 Diabetes Induce Endothelial Dysfunction Via Arginase I. Journal of the American College of Cardiology, 72(7), 769-780. https://doi.org/10.1016/j.jacc.2018.05.052

Erythrocytes From Patients With Type 2 Diabetes Induce Endothelial Dysfunction Via Arginase I. / Zhou, Zhichao; Mahdi, Ali; Tratsiakovich, Yahor; Zahorán, Szabolcs; Kövamees, Oskar; Nordin, Filip; Uribe Gonzalez, Arturo Eduardo; Alvarsson, Michael; Östenson, Claes Göran; Andersson, Daniel C.; Hedin, Ulf; Hermesz, E.; Lundberg, Jon O.; Yang, Jiangning; Pernow, John.

In: Journal of the American College of Cardiology, Vol. 72, No. 7, 14.08.2018, p. 769-780.

Research output: Contribution to journalArticle

Zhou, Z, Mahdi, A, Tratsiakovich, Y, Zahorán, S, Kövamees, O, Nordin, F, Uribe Gonzalez, AE, Alvarsson, M, Östenson, CG, Andersson, DC, Hedin, U, Hermesz, E, Lundberg, JO, Yang, J & Pernow, J 2018, 'Erythrocytes From Patients With Type 2 Diabetes Induce Endothelial Dysfunction Via Arginase I', Journal of the American College of Cardiology, vol. 72, no. 7, pp. 769-780. https://doi.org/10.1016/j.jacc.2018.05.052
Zhou, Zhichao ; Mahdi, Ali ; Tratsiakovich, Yahor ; Zahorán, Szabolcs ; Kövamees, Oskar ; Nordin, Filip ; Uribe Gonzalez, Arturo Eduardo ; Alvarsson, Michael ; Östenson, Claes Göran ; Andersson, Daniel C. ; Hedin, Ulf ; Hermesz, E. ; Lundberg, Jon O. ; Yang, Jiangning ; Pernow, John. / Erythrocytes From Patients With Type 2 Diabetes Induce Endothelial Dysfunction Via Arginase I. In: Journal of the American College of Cardiology. 2018 ; Vol. 72, No. 7. pp. 769-780.
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AU - Zhou, Zhichao

AU - Mahdi, Ali

AU - Tratsiakovich, Yahor

AU - Zahorán, Szabolcs

AU - Kövamees, Oskar

AU - Nordin, Filip

AU - Uribe Gonzalez, Arturo Eduardo

AU - Alvarsson, Michael

AU - Östenson, Claes Göran

AU - Andersson, Daniel C.

AU - Hedin, Ulf

AU - Hermesz, E.

AU - Lundberg, Jon O.

AU - Yang, Jiangning

AU - Pernow, John

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N2 - Background: Cardiovascular complications are major clinical problems in type 2 diabetes mellitus (T2DM). The authors previously demonstrated a crucial role of red blood cells (RBCs) in control of cardiac function through arginase-dependent regulation of nitric oxide export from RBCs. There is alteration of RBC function, as well as an increase in arginase activity, in T2DM. Objectives: The authors hypothesized that RBCs from patients with T2DM induce endothelial dysfunction by up-regulation of arginase. Methods: RBCs were isolated from patients with T2DM and age-matched healthy subjects and were incubated with rat aortas or human internal mammary arteries from nondiabetic patients for vascular reactivity and biochemical studies. Results: Arginase activity and arginase I protein expression were elevated in RBCs from patients with T2DM (T2DM RBCs) through an effect induced by reactive oxygen species (ROS). Co-incubation of arterial segments with T2DM RBCs, but not RBCs from age-matched healthy subjects, significantly impaired endothelial function but not smooth muscle cell function in both healthy rat aortas and human internal mammary arteries. Endothelial dysfunction induced by T2DM RBCs was prevented by inhibition of arginase and ROS both at the RBC and vascular levels. T2DM RBCs induced increased vascular arginase I expression and activity through an ROS-dependent mechanism. Conclusions: This study demonstrates a novel mechanism behind endothelial dysfunction in T2DM that is induced by RBC arginase I and ROS. Targeting arginase I in RBCs may serve as a novel therapeutic tool for the treatment of endothelial dysfunction in T2DM.

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