Epstein-Barr virus permissively infects human syncytiotrophoblasts in vitro and induces replication of human T cell leukemia-lymphoma virus type I in dually infected cells

F. Tóth, George Aboagye-Mathiesen, József Nemes, Xiangdong Liu, István Andirkó, Henrik Hager, Milan Zdravkovic, J. Szabó, Jolán Kiss, János Aranyosi, Peter Ebbesen

Research output: Contribution to journalArticle

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Abstract

Epstein Barr virus (EBV) and human immunodeficiency virus type 1 (HIV-1), as well as human T-cell leukemia-lymphoma virus type I (HTLV-I), may interact in the pathogenesis of human retroviral infections. The placental syncytiotrophoblast layer represents a barrier protecting the fetal compartment from exposure to retroviruses. We studied the interactions of EBV with HIV-1 and HTLV-I in human term syncytiotrophoblast cells to investigate the significance of double infections in transplacental transmission of human retroviruses. We found that syncytiotrophoblast cells could be productively infected with EBV. Dual infection of the cells with EBV and HTLV-I resulted in full replication cycle of otherwise latent HTLV-I. In contrast, the restricted permissiveness of syncytiotrophoblasts for HIV-1 was not influenced by coinfection of the cells with EBV. Infection of syncytiotrophoblast cells with EBV, but not HTLV-I, induced interleukin-2 and interleukin-6 secretion, and augmented secretion occurred on coinfection with both viruses. Coinfection of syncytiotrophoblast cells with EBV and HTLV-I induced tumor necrosis factor-β1 and transforming growth factor-β1 secretion, but infection with either virus alone did not lead to secretion of these cytokines. Permissive replication cycle of HTLV-I was induced by the EBV immediate-early gene product Zta. Pseudotype formation between EBV and HTLV-I in coinfected syncytiotrophoblast cells was not found. Our data suggest that activation of HTLV-I gene expression by EBV in coinfected syncytiotrophoblast cells may be a mechanism for transplacental transmission of HTLV-I.

Original languageEnglish
Pages (from-to)400-414
Number of pages15
JournalVirology
Volume229
Issue number2
DOIs
Publication statusPublished - Mar 17 1997

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Deltaretrovirus
Human T-lymphotropic virus 1
Trophoblasts
Human Herpesvirus 4
Coinfection
HIV-1
Infection
Retroviridae
Permissiveness
In Vitro Techniques
Viruses
Immediate-Early Genes
Transforming Growth Factors
Interleukin-2
Interleukin-6
Tumor Necrosis Factor-alpha
Cytokines

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Epstein-Barr virus permissively infects human syncytiotrophoblasts in vitro and induces replication of human T cell leukemia-lymphoma virus type I in dually infected cells. / Tóth, F.; Aboagye-Mathiesen, George; Nemes, József; Liu, Xiangdong; Andirkó, István; Hager, Henrik; Zdravkovic, Milan; Szabó, J.; Kiss, Jolán; Aranyosi, János; Ebbesen, Peter.

In: Virology, Vol. 229, No. 2, 17.03.1997, p. 400-414.

Research output: Contribution to journalArticle

Tóth, F. ; Aboagye-Mathiesen, George ; Nemes, József ; Liu, Xiangdong ; Andirkó, István ; Hager, Henrik ; Zdravkovic, Milan ; Szabó, J. ; Kiss, Jolán ; Aranyosi, János ; Ebbesen, Peter. / Epstein-Barr virus permissively infects human syncytiotrophoblasts in vitro and induces replication of human T cell leukemia-lymphoma virus type I in dually infected cells. In: Virology. 1997 ; Vol. 229, No. 2. pp. 400-414.
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AU - Liu, Xiangdong

AU - Andirkó, István

AU - Hager, Henrik

AU - Zdravkovic, Milan

AU - Szabó, J.

AU - Kiss, Jolán

AU - Aranyosi, János

AU - Ebbesen, Peter

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