Epstein-Barr virus-infected B-chronic lymphocyte leukemia cells express the virally encoded nuclear proteins but they do not enter the cell cycle

Norihiro Teramoto, Péter Gogolák, Noémi Nagy, Akihiko Maeda, Karin Kvarnung, Magnus Björkholm, Eva Klein

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Objectives: To understand the mechanism for the refractoriness of B- chronic lymphocyte leukemia (B-CLL) cells for EBV-induced immortalization. Study Design/Methods: Cells from four B-CLL patients were infected with Epstein-Barr virus (EBV). Noninfected and infected aliquots were exposed to CD40L. Five days later, the cultures were analyzed for cell survival, activation, DNA synthesis, and expression of EBV-encoded and of cellular regulatory proteins retinoblastoma (Rb), p53, recombinant sequence binding protein (RBP)Jk, and PU.1. The proteins were detected by immunoblotting and by immunofluorescence. Results: A proportion of the cells were activated and ex- pressed Epstein-Barr nuclear antigens (EBNAs) and elevated Rb level but not latent membrane protein (LMP)-1 and p53. They did not enter the cell cycle. Exposure to CD40L induced DNA synthesis but it did not modify the expression of the EBNAs. Conclusions: The virus could activate CLL cells, but the full course of the early events that leads to immortalization - as seen in normal B cells - did not proceed beyond a certain point. Compared to B lymphocytes, the critical point is between activation and initiation of the cell cycle. (C) Lippincott Williams and Wilkins, Inc.

Original languageEnglish
Pages (from-to)125-136
Number of pages12
JournalJournal of Human Virology
Volume3
Issue number3
Publication statusPublished - May 1 2000

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Keywords

  • B-CLL
  • CD40 ligand
  • EBV
  • Rb
  • p53

ASJC Scopus subject areas

  • Virology

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