Epstein-barr virus (HHV-4) inoculation to rabbits by intranasal and oral routes results in subacute and/or persistent infection dissimilar to human disease

Julius Rajčáni, Kalman Szenthe, Vladimira Ďurmanová, Agnes Tóth, Balazs Ásványi, Ervin Pitlik, L. Stipkovits, Susan Szathmary

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Objective: We report the infection of New Zealand white rabbits with Epstein-Barr virus (EBV). Methods: EBV prepared in B95-8 (producer) cells was inoculated to rabbits by combined intranasal and oral routes. Blood and white blood cell (WBC) samples were taken before infection, then on days 8, 28 and 98 post-infection (p.i.). Results: Administration of either 3 × 10 8 (group A, 11 rabbits) or 1 × 109 (group B, 10 rabbits) EBV DNA copies per animal induced subacute and/or persistent infection. The IgG antibodies in plasma were detected by ELISA as well as by immunoblot (IB). The IB bands showed mainly antibodies to the BZRF1/Zta transactivation polypeptide (69.2%), the p54 early protein (53.4%) and to the p23 capsid protein (35.8%). No anti-EBNA1 antibody was detected throughout. Viral DNA could be detected by PCR in WBCs and/or spleen of 7 out of 21 infected rabbits (30%), while 60-80% of them showed serologic response. The transiently present EBV DNA was accompanied by LMP1 antigen. Conclusions: Rabbits developed persistent EBV infection in the absence of EBNA1 antibodies and by the lack of typical infectious mononucleosis-like syndrome. The absence of EBNA1 antibody may reflect the lack of EBNA1 in B cells of EBV-inoculated rabbits.

Original languageEnglish
Pages (from-to)254-269
Number of pages16
JournalIntervirology
Volume57
Issue number5
DOIs
Publication statusPublished - 2014

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Human Herpesvirus 4
Rabbits
Infection
Antibodies
Infectious Mononucleosis
Epstein-Barr Virus Infections
DNA
Viral DNA
Capsid Proteins
Transcriptional Activation
Anti-Idiotypic Antibodies
Leukocytes
B-Lymphocytes
Spleen
Immunoglobulin G
Enzyme-Linked Immunosorbent Assay
Antigens
Polymerase Chain Reaction
Peptides
Proteins

Keywords

  • Epstein-Barr virus
  • Infection markers
  • Rabbit model

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases
  • Medicine(all)

Cite this

Epstein-barr virus (HHV-4) inoculation to rabbits by intranasal and oral routes results in subacute and/or persistent infection dissimilar to human disease. / Rajčáni, Julius; Szenthe, Kalman; Ďurmanová, Vladimira; Tóth, Agnes; Ásványi, Balazs; Pitlik, Ervin; Stipkovits, L.; Szathmary, Susan.

In: Intervirology, Vol. 57, No. 5, 2014, p. 254-269.

Research output: Contribution to journalArticle

Rajčáni, Julius ; Szenthe, Kalman ; Ďurmanová, Vladimira ; Tóth, Agnes ; Ásványi, Balazs ; Pitlik, Ervin ; Stipkovits, L. ; Szathmary, Susan. / Epstein-barr virus (HHV-4) inoculation to rabbits by intranasal and oral routes results in subacute and/or persistent infection dissimilar to human disease. In: Intervirology. 2014 ; Vol. 57, No. 5. pp. 254-269.
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