Epstein-Barr virus can infect B-chronic lymphocytic leukemia cells but it does not orchestrate the cell cycle regulatory proteins

Akihiko Maeda, Kentaro Bandobashi, Noémi Nagy, Norihiro Teramoto, Péter Gogolák, Katja Pokrovskaja, László Székely, Magnus Björkholm, George Klein, Eva Klein

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18 Citations (Scopus)

Abstract

Objectives: To understand the mechanism for the refractoriness of B-chronic lymphocytic leukemia (B-CLL) cells for Epstein-Barr virus (EBV)-induced immortalization. Study Design/Methods: Cultures were initiated with EBV-infected tonsillar B and B-CLL cells. Expression of EBNA-2 and some of the key players regulating G1/S phase transition such as c-myc expression, phosphorylation of Rb protein, expression of G1 cyclins, and the cyclin-dependent kinase inhibitor p27 were followed. Results: In line with earlier studies, EBV infection induced c-myc expression, pRb phosphorylation, D2 and D3 expression, and disappearance of p27 in normal B cells. In contrast, EBV-infected B-CLL cells remained resting and they did not express c-myc; cyclin D2, ppRb and cyclin D3 were seen only in occasional cells. Importantly, p27 expression was maintained. Conclusions: In B-CLL cells, the expression of the EBV-encoded nuclear proteins EBNAs is not followed by entrance to the cell cycle. Thus, the difference in the interaction of EBV-normal B cells and EBV- B-CLL cells is already apparent early after infection.

Original languageEnglish
Pages (from-to)227-237
Number of pages11
JournalJournal of Human Virology
Volume4
Issue number5
Publication statusPublished - Dec 1 2001

Keywords

  • B-CLL
  • C-myc
  • CDK inhibitor
  • Cyclin D2
  • Cyclin D3
  • EBV
  • P27
  • Rb

ASJC Scopus subject areas

  • Virology

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    Maeda, A., Bandobashi, K., Nagy, N., Teramoto, N., Gogolák, P., Pokrovskaja, K., Székely, L., Björkholm, M., Klein, G., & Klein, E. (2001). Epstein-Barr virus can infect B-chronic lymphocytic leukemia cells but it does not orchestrate the cell cycle regulatory proteins. Journal of Human Virology, 4(5), 227-237.