EPR Spectra of spin-labeled fatty acids in detergent solutions: "Immobilized species" in the absence of protein

Stefan Kuntz, Fabian Leinisch, K. Hideg, Wolfgang E. Trommer

Research output: Contribution to journalArticle

Abstract

Electron paramagnetic resonance spectra of 5-doxyl-stearic acid, 3-(2-Dodecyl-1-oxyl-5,5-dimethylpyrrolidin-2-yl)propanoic acid radical (4-proxyl-palmitic acid) and of 3-(1-Oxyl-2,5-dimethyl-5-dodecylpyrrolidine-2-yl)propanoic acid radical (4-azetoxyl-stearic acid) in lipid micelles of nonaethyleneglycol-monododecyl ether (C12E9) and pentaethyleneglycol-monooctyl ether (C8E5) can reveal immobilized components typical of spin-labeled protein-bound fatty acids as well as phospholipids. The occurrence of such species depends on the detergent, the relative orientation of the nitroxide group with respect to the longitudinal axis and the molecular structure of the fatty acid as well as on polarity and ionic strength of the aqueous phase. Whereas 5-doxyl-stearic acid and 4-proxyl-palmitic acid exhibited these highly immobilized components in C12E9 micelles but not in C8E5, only a single relatively mobile component was observed with 4-azetoxyl-stearic acid in both detergents. The immobilized component is lost by the addition of isopropanol but not as much by ethanol and also when distilled water is substituted for the buffer.

Original languageEnglish
Pages (from-to)455-461
Number of pages7
JournalApplied Magnetic Resonance
Volume37
Issue number1
DOIs
Publication statusPublished - 2010

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detergents
fatty acids
proteins
acids
palmitic acid
ethers
micelles
lipids
polarity
electron paramagnetic resonance
ethyl alcohol
molecular structure
buffers
occurrences
water

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

Cite this

EPR Spectra of spin-labeled fatty acids in detergent solutions : "Immobilized species" in the absence of protein. / Kuntz, Stefan; Leinisch, Fabian; Hideg, K.; Trommer, Wolfgang E.

In: Applied Magnetic Resonance, Vol. 37, No. 1, 2010, p. 455-461.

Research output: Contribution to journalArticle

Kuntz, Stefan ; Leinisch, Fabian ; Hideg, K. ; Trommer, Wolfgang E. / EPR Spectra of spin-labeled fatty acids in detergent solutions : "Immobilized species" in the absence of protein. In: Applied Magnetic Resonance. 2010 ; Vol. 37, No. 1. pp. 455-461.
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