Epithelial Ca2+ channel (ECAC1) in autosomal dominant idiopathic hypercalciuria

Dominik Müller, Joost G J Hoenderop, Rudi Vennekens, Paul Eggert, Ferenc Harangi, K. Méhes, Victor Garcia-Nieto, Felix Claverie-Martin, Carel H. Van Os, Bernd Nilius, René J M Bindels

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Background. The epithelial Ca2+ channel (ECaC) exhibits the defining properties for being the gatekeeper in 1,25-dihydroxyvitamin D3-regulated Ca2+ (re)absorption. Its recently cloned human orthologue (ECaC1) could, therefore, represent a crucial molecule in human disorders related to Ca2+-wasting such as idiopathic hypercalciuria (IH). Methods. Fifty-seven members of nine families with IH were investigated. Phenotyping was performed by measurements of urinary Ca2+ excretion, while other underlying disorders were appropriately excluded. Initially, the recently suggested locus for kidney stoneas-sociated hypercalciuria on chromosome 1q23.3-q24 was investigated. Next, direct mutation analysis of all 15 exons of the ECAC1 gene and 2.9 kb upstream from the start codon was performed. hECaC1, heterologously expressed in human embryonic kidney 293 cells, was characterized by patch-clamp analysis. Results. The mode of inheritance in the studied pedigrees is consistent with an autosomal dominant trait. Haplotype analysis did not implicate a role of the locus on chromosome 1. The coding sequence of the ECAC1 gene was not different between the affected and the non-affected family members. In the 5′-flanking region, three single nucleotide polymorphisms were encountered, but these polymorphisms were observed regardless of the affection status of the screened family members. Patch-clamp analysis of hECaC1 was performed as the putative pore region contains four non-conserved amino acid substitutions compared with the other species. This analysis revealed the distinctive properties of ECaC, includin a high Ca2+ selectivity, inward rectification, and C2+ -dependent inactivation. Conclusion. These results do not support a primary role for hECaC1 in IH in nine affected families. Because of the heterogeneity of the disease, however, the involvement of ECaC1 in other subtypes of IH cannot be excluded and needs further investigation. The electrophysiological properties of hECaC1 further substantiate its prime role in Ca2+ (re)absorption.

Original languageEnglish
Pages (from-to)1614-1620
Number of pages7
JournalNephrology Dialysis Transplantation
Volume17
Issue number9
Publication statusPublished - Sep 2002

Fingerprint

Hypercalciuria
Kidney
Initiator Codon
Calcitriol
5' Flanking Region
Chromosomes, Human, Pair 1
Amino Acid Substitution
Pedigree
Haplotypes
Genes
Single Nucleotide Polymorphism
Exons
Chromosomes
Mutation

Keywords

  • Calcium reabsorption
  • ECaC
  • Kidney
  • Patch-clamp
  • TRPV5

ASJC Scopus subject areas

  • Nephrology
  • Transplantation

Cite this

Müller, D., Hoenderop, J. G. J., Vennekens, R., Eggert, P., Harangi, F., Méhes, K., ... Bindels, R. J. M. (2002). Epithelial Ca2+ channel (ECAC1) in autosomal dominant idiopathic hypercalciuria. Nephrology Dialysis Transplantation, 17(9), 1614-1620.

Epithelial Ca2+ channel (ECAC1) in autosomal dominant idiopathic hypercalciuria. / Müller, Dominik; Hoenderop, Joost G J; Vennekens, Rudi; Eggert, Paul; Harangi, Ferenc; Méhes, K.; Garcia-Nieto, Victor; Claverie-Martin, Felix; Van Os, Carel H.; Nilius, Bernd; Bindels, René J M.

In: Nephrology Dialysis Transplantation, Vol. 17, No. 9, 09.2002, p. 1614-1620.

Research output: Contribution to journalArticle

Müller, D, Hoenderop, JGJ, Vennekens, R, Eggert, P, Harangi, F, Méhes, K, Garcia-Nieto, V, Claverie-Martin, F, Van Os, CH, Nilius, B & Bindels, RJM 2002, 'Epithelial Ca2+ channel (ECAC1) in autosomal dominant idiopathic hypercalciuria', Nephrology Dialysis Transplantation, vol. 17, no. 9, pp. 1614-1620.
Müller D, Hoenderop JGJ, Vennekens R, Eggert P, Harangi F, Méhes K et al. Epithelial Ca2+ channel (ECAC1) in autosomal dominant idiopathic hypercalciuria. Nephrology Dialysis Transplantation. 2002 Sep;17(9):1614-1620.
Müller, Dominik ; Hoenderop, Joost G J ; Vennekens, Rudi ; Eggert, Paul ; Harangi, Ferenc ; Méhes, K. ; Garcia-Nieto, Victor ; Claverie-Martin, Felix ; Van Os, Carel H. ; Nilius, Bernd ; Bindels, René J M. / Epithelial Ca2+ channel (ECAC1) in autosomal dominant idiopathic hypercalciuria. In: Nephrology Dialysis Transplantation. 2002 ; Vol. 17, No. 9. pp. 1614-1620.
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AU - Méhes, K.

AU - Garcia-Nieto, Victor

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