Epithelial and endothelial barriers in the olfactory region of the nasal cavity of the rat

Hartwig Wolburg, Karen Wolburg-Buchholz, Heike Sam, Sándor Horvát, M. Deli, Andreas F. Mack

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

The olfactory ensheathing (glial) cells (OECs) have been identified to be useful candidate cells to support regeneration after being transplanted into injured fiber tracts of the central nervous system. We investigated by means of immunocytochemistry and freeze-fracturing the morphology and molecular composition of OEC tight junctions in the rat olfactory system. In addition, we tested the hypothesis whether tight junctions and orthogonal arrays of particles (OAPs) which contain the water channel protein aquaporin-4 (AQP4), are mutually exclusive as suggested in previous studies. In OECs, we found neither OAPs nor AQP4, but tight junctions immunoreactive for ZO-1, occludin, and claudin-5, but immunonegative for ZO-2 and claudin-3. To shed more light on the function of OEC tight junctions, we tested the permeability and tight junction composition of blood vessels and fila olfactoria. We found them both, permeable for infused lanthanum nitrate, and to be immunopositive for ZO-1 and claudin-5. The tight junctions of the OECs are discussed to be responsible for micro- compartmentalization within the olfactory fiber tract providing a benefit for axonal growth.

Original languageEnglish
Pages (from-to)127-140
Number of pages14
JournalHistochemistry and Cell Biology
Volume130
Issue number1
DOIs
Publication statusPublished - Jul 2008

Fingerprint

Tight Junctions
Nasal Cavity
rats
Rats
cavities
Fibers
Blood vessels
Neurology
Lanthanum
Claudin-5
Chemical analysis
Aquaporin 4
Nitrates
Proteins
Claudin-3
Freeze Fracturing
Olfactory Nerve
Occludin
Water
Aquaporins

Keywords

  • Aquaporins
  • Freeze-fracturing
  • Olfactory ensheathing cells
  • Olfactory system
  • Tight junctions

ASJC Scopus subject areas

  • Cell Biology
  • Instrumentation

Cite this

Epithelial and endothelial barriers in the olfactory region of the nasal cavity of the rat. / Wolburg, Hartwig; Wolburg-Buchholz, Karen; Sam, Heike; Horvát, Sándor; Deli, M.; Mack, Andreas F.

In: Histochemistry and Cell Biology, Vol. 130, No. 1, 07.2008, p. 127-140.

Research output: Contribution to journalArticle

Wolburg, Hartwig ; Wolburg-Buchholz, Karen ; Sam, Heike ; Horvát, Sándor ; Deli, M. ; Mack, Andreas F. / Epithelial and endothelial barriers in the olfactory region of the nasal cavity of the rat. In: Histochemistry and Cell Biology. 2008 ; Vol. 130, No. 1. pp. 127-140.
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