Epinephrine and glucagon counteract inhibition of protein synthesis induced by d-galactosamine in isolated mouse hepatocytes

J. Mandl, T. Garzó, K. Mészáros, F. Antoni

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

10 mM d-galactosamine inhibited protein synthesis (1 h incubation time) by 67% in isolated mouse liver cells. Counteracting uridylate deficiency induced by d-galactosamine by preventive administration of 20 mM uridine did not decrease the extent of protein synthesis inhibition. 20 mM d-galactose reverted the inhibition of protein synthesis by d-galactosamine. 10-5M epinephrine and 10-7 M glucagon decreased the incorporation of d-galactosamine into glycogen to 38% and 26% of the control value, respectively, after a 35 min incubation and reduced the inhibition of protein synthesis by d-glactosamine effectively. Experimental evidence supports the view that aminoglycogen formed after d-galactosamine treatment is responsible for the inhibition of protein synthesis.

Original languageEnglish
Pages (from-to)560-567
Number of pages8
JournalBBA - General Subjects
Volume586
Issue number3
DOIs
Publication statusPublished - Sep 3 1979

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Galactosamine
Glucagon
Epinephrine
Hepatocytes
Proteins
Uridine
Glycogen
Galactose
Liver

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology
  • Medicine(all)

Cite this

Epinephrine and glucagon counteract inhibition of protein synthesis induced by d-galactosamine in isolated mouse hepatocytes. / Mandl, J.; Garzó, T.; Mészáros, K.; Antoni, F.

In: BBA - General Subjects, Vol. 586, No. 3, 03.09.1979, p. 560-567.

Research output: Contribution to journalArticle

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